D-Galactose as an autoinducer 2 inhibitor to control the biofilm formation of periodontopathogens
DC Field | Value | Language |
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dc.contributor.author | Ryu, Eun-Ju | - |
dc.contributor.author | Sim, Jaehyun | - |
dc.contributor.author | Sim, Jun | - |
dc.contributor.author | Lee, Julian | - |
dc.contributor.author | Choi, Bong-Kyu | - |
dc.date.available | 2018-05-09T02:25:42Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2016-09 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/7514 | - |
dc.description.abstract | Autoinducer 2 (AI-2) is a quorum sensing molecule to which bacteria respond to regulate various phenotypes, including virulence and biofilm formation. AI-2 plays an important role in the formation of a subgingival biofilm composed mostly of Gram-negative anaerobes, by which periodontitis is initiated. The aim of this study was to evaluate D-galactose as an inhibitor of AI-2 activity and thus of the biofilm formation of periodontopathogens. In a search for an AI-2 receptor of Fusobacterium nucleatum, D-galactose binding protein (Gbp, Gene ID FN1165) showed high sequence similarity with the ribose binding protein (RbsB), a known AI-2 receptor of Aggregatibacter actinomycetemcomitans. D-Galactose was evaluated for its inhibitory effect on the AI-2 activity of Vibrio harveyi BB152 and F. nucleatum, the major coaggregation bridge organism, which connects early colonizing commensals and late pathogenic colonizers in dental biofilms. The inhibitory effect of D-galactose on the biofilm formation of periodontopathogens was assessed by crystal violet staining and confocal laser scanning microscopy in the absence or presence of AI-2 and secreted molecules of F. nucleatum. D-Galactose significantly inhibited the AI-2 activity of V. harveyi and F. nucleatum. In addition, D-galactose markedly inhibited the biofilm formation of F. nucleatum, Porphyromonas gingivalis, and Tannerella forsythia induced by the AI-2 of F. nucleatum without affecting bacterial growth. Our results demonstrate that the Gbp may function as an AI-2 receptor and that galactose may be used for prevention of the biofilm formation of periodontopathogens by targeting AI-2 activity. | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY | - |
dc.subject | FUSOBACTERIUM-NUCLEATUM PK1594 | - |
dc.subject | PERIODONTAL PATHOGENS | - |
dc.subject | BINDING ADHESIN | - |
dc.subject | BACTERIA | - |
dc.subject | ACTINOMYCETEMCOMITANS | - |
dc.subject | COAGGREGATION | - |
dc.subject | MOLECULE | - |
dc.subject | SIGNAL | - |
dc.subject | FAMILY | - |
dc.subject | GROWTH | - |
dc.title | D-Galactose as an autoinducer 2 inhibitor to control the biofilm formation of periodontopathogens | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12275-016-6345-8 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.54, no.9, pp.632 - 637 | - |
dc.identifier.kciid | ART002134854 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000382411700008 | - |
dc.identifier.scopusid | 2-s2.0-84984653765 | - |
dc.citation.endPage | 637 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 632 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 54 | - |
dc.contributor.affiliatedAuthor | Lee, Julian | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | quorum sensing | - |
dc.subject.keywordAuthor | periodontopathogens | - |
dc.subject.keywordAuthor | biofilm | - |
dc.subject.keywordAuthor | AI-2 inhibitor | - |
dc.subject.keywordAuthor | D-galactose | - |
dc.subject.keywordPlus | FUSOBACTERIUM-NUCLEATUM PK1594 | - |
dc.subject.keywordPlus | PERIODONTAL PATHOGENS | - |
dc.subject.keywordPlus | BINDING ADHESIN | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | ACTINOMYCETEMCOMITANS | - |
dc.subject.keywordPlus | COAGGREGATION | - |
dc.subject.keywordPlus | MOLECULE | - |
dc.subject.keywordPlus | SIGNAL | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | GROWTH | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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