Sphingosinicella soli sp nov., isolated from an alkaline soil in Korea

Abstract

A Gram-negative, motile, rod-shaped bacterial strain, KSL-125(T), was isolated from an alkaline soil from Kwangchun, Korea, and its taxonomic position was investigated in a polyphasic study. Strain KSL-125(T) grew optimally at 30 degrees C, at pH 7.5 - 8.0 and in the presence of 0.5% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain KSL-125(T) joins the cluster comprising the two Sphingosinicella species at a bootstrap resampling value of 100%. The values for similarity between the 16S rRNA gene sequence of strain KSL-125(T) and those of the type strains of the two Sphingosinicella species were 98.9 - 99.0%. Strain KSL-125(T) contained Q-10 as the predominant ubiquinone and C-17:1 omega 6c, C-16:1 omega 7c and/or iso-C-15:0 2-OH and C-18:1 omega 7c as the major fatty acids. The major polar lipids were sphingoglycolipid, diphosphaticlylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and an unidentified phospholipid. The DNA G+C content was 65.1 mol%. Strain KSL-125(T) was distinguishable from the two recognized Sphingosinicella species on the basis of differential phenotypic properties, DNA - DNA relatedness data and repetitive-sequence-based PCR genomic fingerprinting patterns. The phenotypic, phylogenetic and genetic data showed that strain KSL-125(T) represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella soli sp. nov. is proposed. The type strain is KSL-125(T) (=KCTC 12482(T) =DSM 17328(T)).