Pseudoclavibacter chungangensis sp nov., isolated from activated sludge

Abstract

The taxonomic position of a Gram-positive, non-spore-forming strain, designated CAU 59(T), from activated sludge was investigated. Colony morphology, biochemical tests and chemotaxonomic investigations revealed that strain CAU 59T possessed the characteristics of the genus Pseudoclavibacter. Comparative 16S rRNA gene sequence analysis showed sequence divergence values between strain CAU 59T and other described Pseudoclavibacter species of more than 3.6%, and the strain formed a hitherto-unknown subline within the genus Pseudoclavibacter. DNA-DNA hybridization studies showed that strain CAU 59T displayed 20.9% relatedness to its closest phylogenetic neighbour, Pseudoclavibacter helvolus DSM 20419(T.) The DNA G+C content was 66.2 mol%. The phenotypic, chemotaxonomic and genotypic data indicated that strain CAU 59T represents a novel species of the genus Pseudoclavibacter, for which the name Pseudoclavibacter chungangensis sp. nov. is proposed. The type strain is CAU 59T (=KCTC 22691(T) = CCUG 58142(T)).