수종 생약이 일차배양한 계배의 뇌세포에 미치는 영향Studies on the Effect of Several Crude Drugs on Cultured Chicken Brain Cells
- Authors
- 박미정; 송진호; 김영중
- Issue Date
- Mar-1989
- Publisher
- 한국생약학회
- Keywords
- Lycium chinensis; Epimedium koreanum Aconitum sibiricum; tuguaco; nitine; chicken embryo; brain cell culture; pyruvate dehydrogenase complex
- Citation
- 생약학회지, v.20, no.1, pp 32 - 36
- Pages
- 5
- Journal Title
- 생약학회지
- Volume
- 20
- Number
- 1
- Start Page
- 32
- End Page
- 36
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/66639
- ISSN
- 0253-3073
- Abstract
- Effects of Lycium chinensis, Epimedium koreanum and tuguaconitine which is isolated from Aconitum sibiricum on primary cultured chicken embryonic brain cells were studied by microscopic observation and determination of the activity of pyruvate dehydrogenase complex(PDHC). Brain cells were prepared from the brain of 10-day-old chicken embryo and cultured with a medium consisted of 90% Dulbec co`s Modified Eagle Medium(DMEM) and 10% horse serum. It was observed that all substances studied seemed to show the tendency to stimulate the neurite outgrowth of brain cells which were cultured with a deficient medium under microscopic observation. The activity of PDHC in brain cells cultured with a deficient medium was increased by Lycium chinensis and Epimedium koreanum. However, tuguaconitine had not influence on the activity of PDHC.
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