Structural stability of CD1 domain of human mitotic checkpoint serine/threonine-protein kinase, Bub1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyun-Hwi | - |
dc.contributor.author | Song, Hyun-Kyu | - |
dc.contributor.author | Lee, Bong-Jin | - |
dc.contributor.author | Park, Sung Jean | - |
dc.date.available | 2020-02-28T07:45:16Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.issn | 1226-6531 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10059 | - |
dc.description.abstract | Bub1 is one of the spindle checkpoint proteins and plays a role in recruitment of the related proteins to kinetochore. Here, we studied the structural characteristic of the evolutionarily conserved 160 amino acid region in the N-terminus (hBub1 CD1), using Circular Dichroism (CD) and NMR. Our CD results showed that hBub1 CD1 is a highly helical protein and its structure was affected by pH: as pH was elevated to basic pH, the helical propensity increased. This could be related to the surface charge of the hBub1 CD1. However, the structural change did not largely depend on the salt concentration, though the thermal stability a little increased. The previous NMR analysis1 revealed that the hBub1 CD1 adopts eight helices, which is consistent with the CD result. Our result would be helpful for evaluating the molecular mechanism of the hBub1 CD1 and protein-protein interactions. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN MAGNETIC RESONANCE SOC | - |
dc.relation.isPartOf | JOURNAL OF THE KOREAN MAGNETIC RESONANCE SOCIETY | - |
dc.title | Structural stability of CD1 domain of human mitotic checkpoint serine/threonine-protein kinase, Bub1 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.wosid | 000410031100006 | - |
dc.identifier.doi | 10.6564/JKMRS.2015.19.2.088 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE KOREAN MAGNETIC RESONANCE SOCIETY, v.19, no.2, pp.88 - 94 | - |
dc.identifier.kciid | ART002039928 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 94 | - |
dc.citation.startPage | 88 | - |
dc.citation.title | JOURNAL OF THE KOREAN MAGNETIC RESONANCE SOCIETY | - |
dc.citation.volume | 19 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Kim, Hyun-Hwi | - |
dc.contributor.affiliatedAuthor | Park, Sung Jean | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | NMR | - |
dc.subject.keywordAuthor | CD | - |
dc.subject.keywordAuthor | Bub1 | - |
dc.subject.keywordAuthor | human mitotic checkpoint serine/threonine-protein kinase | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.