Liquid crystal sensor for the detection of acetylcholine using acetylcholinesterase immobilized on a nanostructured polymeric surface
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Gyeo-Re | - |
dc.contributor.author | Jang, Chang-Hyun | - |
dc.date.available | 2020-02-28T07:45:32Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.issn | 0303-402X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10074 | - |
dc.description.abstract | In this study, we report on a label-free biosensor for the detection of acetylcholine (ACh) and an acetylcholinesterase (AChE) inhibitor, utilizing liquid crystals (LCs) on a polymeric surface with periodic nanostructures. The polymeric nanostructures, which hold sinusoidal anisotropic patterns, were fabricated by a sequential process of poly(dimethylsiloxane) buckling and replication of these patterns on a poly(urethane acrylate) surface where a film of gold was deposited. AChE was then covalently immobilized onto the gold surface. Optical images of nematic 4-cyano-4'-pentylbiphenyl (5CB) revealed that it aligned parallel to the plane of the enzyme-decorated surface. However, AChE-mediated hydrolysis of ACh resulted in a plume of choline, acetate, and unreacted ACh, which in turn produced a distinctive optical transition of 5CB (from uniform to random) by masking the anisotropic surface topography. The hydrolysis of ACh was inhibited in the presence of a carbamate insecticide (AChE inhibitor), which prevented the orientational transition of 5CB by decreasing the enzymatic activity of AChE. These results suggest that this LC-based enzymatic sensor is highly sensitive to ACh and the AChE inhibitor, with a detection limit of 10 and 1 nM, respectively. This study demonstrates that polymeric surfaces with continuous wavy features can be used as LC-based biosensors to amplify the existence of ACh and an AChE inhibitor without labeling or an additional amplification strategy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.relation.isPartOf | COLLOID AND POLYMER SCIENCE | - |
dc.subject | GAS-CHROMATOGRAPHY | - |
dc.subject | MASS-SPECTROMETRY | - |
dc.subject | SOLID-SURFACES | - |
dc.subject | CARBOFURAN | - |
dc.subject | BIOSENSOR | - |
dc.subject | INSECTICIDES | - |
dc.subject | INHIBITION | - |
dc.subject | PATTERNS | - |
dc.subject | CHOLINE | - |
dc.subject | TISSUE | - |
dc.title | Liquid crystal sensor for the detection of acetylcholine using acetylcholinesterase immobilized on a nanostructured polymeric surface | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000361568400005 | - |
dc.identifier.doi | 10.1007/s00396-015-3648-y | - |
dc.identifier.bibliographicCitation | COLLOID AND POLYMER SCIENCE, v.293, no.10, pp.2771 - 2779 | - |
dc.identifier.scopusid | 2-s2.0-84942296647 | - |
dc.citation.endPage | 2779 | - |
dc.citation.startPage | 2771 | - |
dc.citation.title | COLLOID AND POLYMER SCIENCE | - |
dc.citation.volume | 293 | - |
dc.citation.number | 10 | - |
dc.contributor.affiliatedAuthor | Han, Gyeo-Re | - |
dc.contributor.affiliatedAuthor | Jang, Chang-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Liquid crystals | - |
dc.subject.keywordAuthor | Polymeric surfaces | - |
dc.subject.keywordAuthor | Acetylcholine | - |
dc.subject.keywordAuthor | Acetylcholinesterase | - |
dc.subject.keywordAuthor | Acetylcholinesterase inhibitor | - |
dc.subject.keywordPlus | GAS-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | SOLID-SURFACES | - |
dc.subject.keywordPlus | CARBOFURAN | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | INSECTICIDES | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | PATTERNS | - |
dc.subject.keywordPlus | CHOLINE | - |
dc.subject.keywordPlus | TISSUE | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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