Osteogenic/Angiogenic Dual Growth Factor Delivery Microcapsules for Regeneration of Vascularized Bone Tissue
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Subbiah, Ramesh | - |
dc.contributor.author | Hwang, Mintai Peter | - |
dc.contributor.author | Van, Se Young | - |
dc.contributor.author | Do, Sun Hee | - |
dc.contributor.author | Park, Hansoo | - |
dc.contributor.author | Lee, Kangwon | - |
dc.contributor.author | Kim, Sang Heon | - |
dc.contributor.author | Yun, Kyusik | - |
dc.contributor.author | Park, Kwideok | - |
dc.date.available | 2020-02-28T08:41:38Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-09-16 | - |
dc.identifier.issn | 2192-2640 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10143 | - |
dc.description.abstract | Growth factors (GFs) are major biochemical cues for tissue regeneration. Herein, a novel dual GF delivery system is designed composed of poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) and alginate microcapsules (MCs) via an electrodropping method. While bone morphogenetic protein (BMP)-2 is encapsulated in the PLGA NPs, vascular endothelial growth factor (VEGF) is included in the alginate MCs, where BMP-2-loaded PLGA NPs are entrapped together in the fabrication process. The initial loading efficiencies of BMP-2 and VEGF are 78% +/- 3.6% and 43% +/- 1.7%, respectively. When our dual GF-loaded MCs are assessed for in vitro osteogenesis of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) on 2D and 3D environment, MCs contribute to much better UCB-MSCs osteogenesis as confirmed by von Kossa staining, immunofluorescence (osteocalcin, collagen 1), calcium content measurement, and osteogenic markers expression. In addition, when dual GF-encapsulated MCs are combined with collagen and then applied to 8 mm diameter rat calvarial defect model, the positive effects on vascularized bone regeneration are much more pronounced; micro computed tomography (CT) and histology analyses exhibit 82.3% bone healing coupled with 12.6% vessel occupied area. Put together, current study indicates a synergistic effect of BMP-2/VEGF and highlights the great potential of dual GF delivery modality (PLGA NPs-in-MC) for regeneration of vascularized bone. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.relation.isPartOf | ADVANCED HEALTHCARE MATERIALS | - |
dc.subject | SIZE DEFECT MODEL | - |
dc.subject | MORPHOGENETIC PROTEIN-2 | - |
dc.subject | STEM-CELLS | - |
dc.subject | BIOMEDICAL APPLICATIONS | - |
dc.subject | OSTEOGENIC RESPONSE | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | VEGF | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | MICROSPHERES | - |
dc.subject | SYSTEM | - |
dc.title | Osteogenic/Angiogenic Dual Growth Factor Delivery Microcapsules for Regeneration of Vascularized Bone Tissue | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000367752300009 | - |
dc.identifier.doi | 10.1002/adhm.201500341 | - |
dc.identifier.bibliographicCitation | ADVANCED HEALTHCARE MATERIALS, v.4, no.13, pp.1982 - 1992 | - |
dc.identifier.scopusid | 2-s2.0-84941670318 | - |
dc.citation.endPage | 1992 | - |
dc.citation.startPage | 1982 | - |
dc.citation.title | ADVANCED HEALTHCARE MATERIALS | - |
dc.citation.volume | 4 | - |
dc.citation.number | 13 | - |
dc.contributor.affiliatedAuthor | Yun, Kyusik | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Bone regeneration | - |
dc.subject.keywordAuthor | Dual growth factor delivery | - |
dc.subject.keywordAuthor | Microcapsules | - |
dc.subject.keywordAuthor | Nanoparticles | - |
dc.subject.keywordAuthor | Umbilical cord, mesenchymal stem cells | - |
dc.subject.keywordPlus | SIZE DEFECT MODEL | - |
dc.subject.keywordPlus | MORPHOGENETIC PROTEIN-2 | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | BIOMEDICAL APPLICATIONS | - |
dc.subject.keywordPlus | OSTEOGENIC RESPONSE | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | VEGF | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | MICROSPHERES | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.