Role of protein kinase C- in cigarette smoke extract-induced apoptosis in MRC-5-cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Son, E. S. | - |
dc.contributor.author | Kyung, S. Y. | - |
dc.contributor.author | Lee, S. P. | - |
dc.contributor.author | Jeong, S. H. | - |
dc.contributor.author | Shin, J. Y. | - |
dc.contributor.author | Ohba, M. | - |
dc.contributor.author | Yeo, E. J. | - |
dc.contributor.author | Park, J. W. | - |
dc.date.available | 2020-02-28T08:42:46Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-09 | - |
dc.identifier.issn | 0960-3271 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10201 | - |
dc.description.abstract | Cigarette smoke (CS) is a major risk factor for emphysema, which causes cell death in structural cells of the lung by mechanisms that are still not completely understood. We demonstrated previously that CS extract (CSE) induces caspase activation in MRC-5 human lung fibroblasts, activated protein kinase C- (PKC-), and translocated PKC- from the cytosol to the membrane. The objective of this study was to investigate the involvement of PKC- activation in a CSE-induced extrinsic apoptotic pathway. We determined that CSE increases expression of caspase 3 and 8 cleavage in MRC-5 cells and overexpression of PKC- significantly increased expression of caspase 3 and 8 cleavage compared with control LacZ-infected cells. In contrast, dominant negative (dn) PKC- inhibited apoptosis in MRC-5 cells exposed to CSE and decreased expression of caspase 3 and 8 compared with control cells. Exposure to 10% CSE for >8 h significantly increased lactate dehydrogenase release in PKC--infected cells compared with LacZ-infected cells. Additionally, PKC--infected cells had an increased number of Hoechst 33342 stained nuclei compared with LacZ-infected cells, while dn PKC--infected cells exhibited fewer morphological changes than LacZ-infected cells under phase-contrast microscopy. In conclusion, PKC- activation plays a pro-apoptotic role in CSE-induced extrinsic apoptotic pathway in MRC-5 cells. These results suggest that modulation of PKC- may be a useful tool for regulating the extrinsic apoptosis of MRC-5 cells by CSE and may have therapeutic potential in the treatment of CS-induced lung injury. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SAGE PUBLICATIONS LTD | - |
dc.relation.isPartOf | HUMAN & EXPERIMENTAL TOXICOLOGY | - |
dc.subject | OBSTRUCTIVE PULMONARY-DISEASE | - |
dc.subject | DNA-DAMAGE | - |
dc.subject | CELL-DEATH | - |
dc.subject | LUNG | - |
dc.subject | ETA | - |
dc.subject | INDUCTION | - |
dc.subject | FORM | - |
dc.title | Role of protein kinase C- in cigarette smoke extract-induced apoptosis in MRC-5-cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000360090300002 | - |
dc.identifier.doi | 10.1177/0960327114561343 | - |
dc.identifier.bibliographicCitation | HUMAN & EXPERIMENTAL TOXICOLOGY, v.34, no.9, pp.869 - 877 | - |
dc.identifier.scopusid | 2-s2.0-84939641251 | - |
dc.citation.endPage | 877 | - |
dc.citation.startPage | 869 | - |
dc.citation.title | HUMAN & EXPERIMENTAL TOXICOLOGY | - |
dc.citation.volume | 34 | - |
dc.citation.number | 9 | - |
dc.contributor.affiliatedAuthor | Son, E. S. | - |
dc.contributor.affiliatedAuthor | Kyung, S. Y. | - |
dc.contributor.affiliatedAuthor | Lee, S. P. | - |
dc.contributor.affiliatedAuthor | Jeong, S. H. | - |
dc.contributor.affiliatedAuthor | Shin, J. Y. | - |
dc.contributor.affiliatedAuthor | Yeo, E. J. | - |
dc.contributor.affiliatedAuthor | Park, J. W. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | chronic obstructive pulmonary disease | - |
dc.subject.keywordAuthor | cigarette smoke | - |
dc.subject.keywordAuthor | protein kinase C | - |
dc.subject.keywordPlus | OBSTRUCTIVE PULMONARY-DISEASE | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | LUNG | - |
dc.subject.keywordPlus | ETA | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | FORM | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.