Leptin Elongates Hypothalamic Neuronal Cilia via Transcriptional Regulation and Actin Destabilization
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Gil Myoung | - |
dc.contributor.author | Han, Yu Mi | - |
dc.contributor.author | Ko, Hyuk Whan | - |
dc.contributor.author | Kim, Joon | - |
dc.contributor.author | Oh, Byung Chul | - |
dc.contributor.author | Kwon, Ijoo | - |
dc.contributor.author | Kim, Min-Seon | - |
dc.date.available | 2020-02-28T08:45:32Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-07-17 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10330 | - |
dc.description.abstract | Terminally differentiated neurons have a single, primary cilium. The primary cilia of hypothalamic neurons play a critical role in sensing metabolic signals. We recently showed that mice with leptin deficiency or resistance have shorter cilia in the hypothalamic neurons, and leptin treatment elongates cilia in hypothalamic neurons. Here, we investigated the molecular mechanisms by which leptin controls ciliary length in hypothalamic neurons. In N1 hypothalamic neuronal cells, leptin treatment increased the expression of intraflagellar transport proteins. These effects occurred via phosphatase and tensin homolog/glycogen synthase kinase-3 beta-mediated inhibition of the transcriptional factor RFX1. Actin filament dynamics were also involved in leptin-promoted ciliary elongation. Both leptin and cytochalasin-D treatment induced F-actin disruption and cilium elongation in hypothalamic neurons that was completely abrogated by co-treatment with the F-actin polymerizer phalloidin. Our findings suggest that leptin elongates hypothalamic neuronal cilia by stimulating the production of intraflagellar transport proteins and destabilizing actin filaments. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.subject | FACTOR RFX3 | - |
dc.subject | CILIOGENESIS | - |
dc.subject | INTRAFLAGELLAR | - |
dc.subject | PROTEINS | - |
dc.subject | INHIBITION | - |
dc.subject | ASYMMETRY | - |
dc.subject | OBESITY | - |
dc.title | Leptin Elongates Hypothalamic Neuronal Cilia via Transcriptional Regulation and Actin Destabilization | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000358511700045 | - |
dc.identifier.doi | 10.1074/jbc.M115.639468 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.290, no.29, pp.18146 - 18155 | - |
dc.identifier.scopusid | 2-s2.0-84937458771 | - |
dc.citation.endPage | 18155 | - |
dc.citation.startPage | 18146 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 290 | - |
dc.citation.number | 29 | - |
dc.contributor.affiliatedAuthor | Oh, Byung Chul | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | FACTOR RFX3 | - |
dc.subject.keywordPlus | CILIOGENESIS | - |
dc.subject.keywordPlus | INTRAFLAGELLAR | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | ASYMMETRY | - |
dc.subject.keywordPlus | OBESITY | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.