A Novel Synthetic Compound, YH-1118, Inhibited LPS-Induced Inflammatory Response by Suppressing I kappa B Kinase/NF-kappa B Pathway in Raw 264.7 Cells
DC Field | Value | Language |
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dc.contributor.author | Yun, Chang Hyun | - |
dc.contributor.author | Jang, Eun Jung | - |
dc.contributor.author | Kwon, Soon Cheon | - |
dc.contributor.author | Lee, Mee-Young | - |
dc.contributor.author | Lee, Sartgku | - |
dc.contributor.author | Oh, Sei-Ryang | - |
dc.contributor.author | Lee, Hyeong-Kyu | - |
dc.contributor.author | Ahn, Kyung-Seop | - |
dc.contributor.author | Lee, Ho-Jae | - |
dc.date.available | 2020-02-28T08:46:44Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10396 | - |
dc.description.abstract | For the search of a potent first-in-class compound to inactivate macrophages responsible for inflammatory responses, in the present study, we investigated the anti-inflammatory effects of YH-1118, a novel synthetic compound, in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line, Raw 264.7. YH-1118 inhibited LPS-induced nitric oxide (NO) production and inducible NO synthase (iNOS) expression at both the protein and mRNA levels. The suppression of LPS-induced iNOS expression by YH-1118 was mediated via nuclear factor kappa B (NF-kappa B), but not activator protein-1 (AP-1) transcription factor. This was supported by the finding that YH-1118 attenuated the phosphorylation of inhibitor of kappa B alpha (I kappa B alpha) and nuclear translocation and DNA binding activity of NF-kappa B. Through the mechanisms that YH-1118 inhibited the activation of I kappa B kinases (IKKs), upstream activators of NF-kappa B, or p38 MAPK, YH-1118 significantly suppressed LPS-induced production of pro-inflammatory cytokines, tumor necrosis factor-alpha, interleukin-1 beta (IL-1 beta), and IL-6 (p < 0.05). In conclusion, our results suggest that YH-1118 inhibits LPS-induced inflammatory responses by blocking IKK and NF-kappa B activation in macrophages, and may be a therapeutic candidate for the treatment of various inflammatory diseases. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.subject | NITRIC-OXIDE SYNTHASE | - |
dc.subject | ACTIVATED PROTEIN-KINASE | - |
dc.subject | INTERFERON-GAMMA | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | IKK-ALPHA | - |
dc.subject | LIPOPOLYSACCHARIDE | - |
dc.subject | INDUCTION | - |
dc.subject | BETA | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | MONOCYTES | - |
dc.title | A Novel Synthetic Compound, YH-1118, Inhibited LPS-Induced Inflammatory Response by Suppressing I kappa B Kinase/NF-kappa B Pathway in Raw 264.7 Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000358701800010 | - |
dc.identifier.doi | 10.4014/jmb.1504.04027 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.25, no.7, pp.1047 - 1055 | - |
dc.identifier.kciid | ART002014176 | - |
dc.identifier.scopusid | 2-s2.0-84937418601 | - |
dc.citation.endPage | 1055 | - |
dc.citation.startPage | 1047 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 25 | - |
dc.citation.number | 7 | - |
dc.contributor.affiliatedAuthor | Yun, Chang Hyun | - |
dc.contributor.affiliatedAuthor | Jang, Eun Jung | - |
dc.contributor.affiliatedAuthor | Kwon, Soon Cheon | - |
dc.contributor.affiliatedAuthor | Lee, Ho-Jae | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | YH-1118 | - |
dc.subject.keywordAuthor | iNOS | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | macrophages | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | INTERFERON-GAMMA | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | IKK-ALPHA | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | BETA | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | MONOCYTES | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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