Characterisation of insulin-producing cells differentiated from tonsil derived mesenchymal stem cells
DC Field | Value | Language |
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dc.contributor.author | Kim, So-Yeon | - |
dc.contributor.author | Kim, Ye-Ryung | - |
dc.contributor.author | Park, Woo-Jae | - |
dc.contributor.author | Kim, Han Su | - |
dc.contributor.author | Jung, Sung-Chul | - |
dc.contributor.author | Woo, So-Youn | - |
dc.contributor.author | Jo, Inho | - |
dc.contributor.author | Ryu, Kyung-Ha | - |
dc.contributor.author | Park, Joo-Won | - |
dc.date.available | 2020-02-28T08:47:02Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 0301-4681 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10409 | - |
dc.description.abstract | Tonsil-derived (T-) mesenchymal stem cells (MSCs) display mutilineage differentiation potential and self-renewal capacity and have potential as a banking source. Diabetes mellitus is a prevalent disease in modern society, and the transplantation of pancreatic progenitor cells or various stem cell-derived insulin-secreting cells has been suggested as a novel therapy for diabetes. The potential of T-MSCs to trans-differentiate into pancreatic progenitor cells or insulin-secreting cells has not yet been investigated. We examined the potential of human T-MSCs to trans-differentiate into pancreatic islet cells using two different methods based on beta-mercaptoethanol and insulin-transferin-selenium, respectively. First, we compared the efficacy of the two methods for inducing differentiation into insulin-producing cells. We demonstrated that the insulin-transferin-selenium method is more efficient for inducing differentiation into insulin-secreting cells regardless of the source of the MSCs. Second, we compared the differentiation potential of two different MSC types: T-MSCs and adipose-derived MSCs (A-MSCs). T-MSCs had a differentiation capacity similar to that of A-MSCs and were capable of secreting insulin in response to glucose concentration. Islet-like clusters differentiated from T-MSCs had lower synaptotagmin-3, -5, -7, and -8 levels, and consequently lower secreted insulin levels than cells differentiated from A-MSCs. These results imply that T-MSCs can differentiate into functional pancreatic islet-like cells and could provide a novel, alternative cell therapy for diabetes mellitus. (C) 2015 International Society of Differentiation. Published by Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.relation.isPartOf | DIFFERENTIATION | - |
dc.subject | PANCREATIC BETA-CELLS | - |
dc.subject | THERAPY POSITION STATEMENT | - |
dc.subject | BONE-MARROW | - |
dc.subject | STROMAL CELLS | - |
dc.subject | IN-VITRO | - |
dc.subject | ADIPOSE-TISSUE | - |
dc.subject | INTERNATIONAL-SOCIETY | - |
dc.subject | DIABETES-MELLITUS | - |
dc.subject | MICE | - |
dc.subject | EXOCYTOSIS | - |
dc.title | Characterisation of insulin-producing cells differentiated from tonsil derived mesenchymal stem cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000365802600003 | - |
dc.identifier.doi | 10.1016/j.diff.2015.08.001 | - |
dc.identifier.bibliographicCitation | DIFFERENTIATION, v.90, no.1-3, pp.27 - 39 | - |
dc.identifier.scopusid | 2-s2.0-84949531463 | - |
dc.citation.endPage | 39 | - |
dc.citation.startPage | 27 | - |
dc.citation.title | DIFFERENTIATION | - |
dc.citation.volume | 90 | - |
dc.citation.number | 1-3 | - |
dc.contributor.affiliatedAuthor | Park, Woo-Jae | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Insulin | - |
dc.subject.keywordAuthor | Diabetes | - |
dc.subject.keywordAuthor | Mesenchymal stem cell | - |
dc.subject.keywordAuthor | Tonsil | - |
dc.subject.keywordAuthor | Adipose tissue | - |
dc.subject.keywordPlus | PANCREATIC BETA-CELLS | - |
dc.subject.keywordPlus | THERAPY POSITION STATEMENT | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | INTERNATIONAL-SOCIETY | - |
dc.subject.keywordPlus | DIABETES-MELLITUS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | EXOCYTOSIS | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Developmental Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Developmental Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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