Bulk Aligner: A novel sequence alignment algorithm based on graph theory and Trinity

Abstract

Sequence alignment is a widely-used tool in genomics. With the development of next generation sequencing (NGS) technology, the production of sequence read data has recently increased. A number of read alignment algorithms for handling NGS data have been developed. However, these algorithms suffer from a trade-off between the throughput and alignment quality, due to the large computational costs for processing repeat reads. Conversely, alignment algorithms with distributed systems such as Hadoop and Trinity can obtain a better throughput than existing algorithms on single machine without compromising the alignment quality. In this paper, we suggest BulkAligner, a novel sequence alignment algorithm on the graph-based in-memory distributed system Trinity. We covert the original reference sequence into graph form and perform sequence alignment by finding the longest paths on the graph. Our experimental results show that BulkAligner has at least an 1.8x and up to 57x better throughput with the same, or higher quality than existing algorithms with Hadoop. We analyze the scalability and show that we can obtain a better throughput by simply adding machines. (C) 2015 Elsevier Inc. All rights reserved.