Potentiometric Urea Biosensor Based on Carbon Nanotubes and Polyion Complex Film
- Authors
- Linh Thi My Nguyen; Yoon, Hyon Hee
- Issue Date
- Feb-2015
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Urea Sensor; Urease; Carbon Nanotube; Polyion Complex
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.15, no.2, pp.1150 - 1153
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 15
- Number
- 2
- Start Page
- 1150
- End Page
- 1153
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10796
- DOI
- 10.1166/jnn.2015.9327
- ISSN
- 1533-4880
- Abstract
- Enzymatic electrode for a potentiometric urea sensor was prepared by sequential coating of carbon nanotube (CNT), urease (Urs) and polyion complex (mixture of poly-L-lysine hydrobromide and poly (sodium 4-styrenesulfonate), PIC) on an ITO glass. The prepared electrode (ITO/CNT/Urs/PIC) was characterized by potentiometric measurements at different urea concentrations in Tris-HCI buffer (pH 7.0). The potentiometric response of the electrode was linear in the range of 1 x 10(-5) to 3 x 10(-3) M with a correlation coefficient of 0.999 and a sensitivity of 59.1 mV/decade. It was found that the addition of CNT caused considerable improvement of the sensitivity of the electrode to urea. The response time was approximately 60-90 s. A half of the initial sensitivity was retained for 15-17 d at room temperature.
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