Co-culture with Mature Islet Cells Augments the Differentiation of Insulin-Producing Cells from Pluripotent Stem Cells
DC Field | Value | Language |
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dc.contributor.author | Oh, Bea Jun | - |
dc.contributor.author | Oh, Seung-Hoon | - |
dc.contributor.author | Choi, Jin Myung | - |
dc.contributor.author | Jin, Sang-Man | - |
dc.contributor.author | Shim, Woo-Young | - |
dc.contributor.author | Lee, Myung-Shik | - |
dc.contributor.author | Lee, Moon-Kyu | - |
dc.contributor.author | Kim, Kwang-Won | - |
dc.contributor.author | Kim, Jae Hyeon | - |
dc.date.available | 2020-02-28T10:43:17Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-02 | - |
dc.identifier.issn | 1550-8943 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10832 | - |
dc.description.abstract | Islet transplantation has been hampered by the shortage of islet donors available for diabetes therapy. However, pluripotent stem cells (PSCs) can be an alternative source of insulin-producing cells (IPCs) because of their capacity for self-renewal and differentiation. We described a method to efficiently differentiate PSCs into IPCs by co-culturing mature islets with directed-differentiated pancreatic endoderm (PE) cells from mouse and human PSCs. PE cells co-cultured with islet cells or islet cell-derived conditioned medium (CM) showed increased expression levels of beta-cell markers; significantly higher levels of proinsulin- and Newport Green (NG)-positive cells, which revealed the characteristics of insulin producing cells; and increased insulin secretion upon glucose stimulation. Co-culturing human PE cells with islet cells was also effective to differentiate PE cells into IPCs. Diabetic nude mice transplanted with co-cultured cells exhibited restored euglycemia, human C-peptide release, and improved glucose tolerance. Immunohistochemistry revealed that insulin+/C-peptide + cells existed in the grafted tissues. These results suggest that mature islet cells can increase the differentiation efficiency of PE cells into mature IPCs via paracrine effects. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | HUMANA PRESS INC | - |
dc.relation.isPartOf | STEM CELL REVIEWS AND REPORTS | - |
dc.subject | PANCREATIC BETA-CELLS | - |
dc.subject | PROGENITOR CELLS | - |
dc.subject | SECRETING CELLS | - |
dc.subject | IPS CELLS | - |
dc.subject | ACTIVIN-A | - |
dc.subject | HUMAN ES | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | REGENERATION | - |
dc.subject | SIGNALS | - |
dc.subject | MICE | - |
dc.title | Co-culture with Mature Islet Cells Augments the Differentiation of Insulin-Producing Cells from Pluripotent Stem Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000349957200006 | - |
dc.identifier.doi | 10.1007/s12015-014-9554-8 | - |
dc.identifier.bibliographicCitation | STEM CELL REVIEWS AND REPORTS, v.11, no.1, pp.62 - 74 | - |
dc.identifier.scopusid | 2-s2.0-84923642794 | - |
dc.citation.endPage | 74 | - |
dc.citation.startPage | 62 | - |
dc.citation.title | STEM CELL REVIEWS AND REPORTS | - |
dc.citation.volume | 11 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Kim, Kwang-Won | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Pluripotent stem cells | - |
dc.subject.keywordAuthor | Insulin-producing cells | - |
dc.subject.keywordAuthor | Co-culture | - |
dc.subject.keywordAuthor | beta-cell differentiation | - |
dc.subject.keywordAuthor | Diabetes | - |
dc.subject.keywordAuthor | Transplantation | - |
dc.subject.keywordPlus | PANCREATIC BETA-CELLS | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | SECRETING CELLS | - |
dc.subject.keywordPlus | IPS CELLS | - |
dc.subject.keywordPlus | ACTIVIN-A | - |
dc.subject.keywordPlus | HUMAN ES | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | SIGNALS | - |
dc.subject.keywordPlus | MICE | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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