Bromine isotopic signature facilitates de novo sequencing of peptides in free-radical-initiated peptide sequencing (FRIPS) mass spectrometry
DC Field | Value | Language |
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dc.contributor.author | Nam, Jungjoo | - |
dc.contributor.author | Kwon, Hyuksu | - |
dc.contributor.author | Jang, Inae | - |
dc.contributor.author | Jeon, Aeran | - |
dc.contributor.author | Moon, Jingyu | - |
dc.contributor.author | Lee, Sun Young | - |
dc.contributor.author | Kang, Dukjin | - |
dc.contributor.author | Han, Sang Yun | - |
dc.contributor.author | Moon, Bongjin | - |
dc.contributor.author | Oh, Han Bin | - |
dc.date.available | 2020-02-28T10:43:26Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-02 | - |
dc.identifier.issn | 1076-5174 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10842 | - |
dc.description.abstract | We recently showed that free-radical-initiated peptide sequencing mass spectrometry (FRIPS MS) assisted by the remarkable thermochemical stability of (2,2,6,6-tetramethyl-piperidin-1-yl)oxyl (TEMPO) is another attractive radical-driven peptide fragmentation MS tool. Facile homolytic cleavage of the bond between the benzylic carbon and the oxygen of the TEMPO moiety in o-TEMPO-Bz-C(O)-peptide and the high reactivity of the benzylic radical species generated in center dot Bz-C(O)-peptide are key elements leading to extensive radical-driven peptide backbone fragmentation. In the present study, we demonstrate that the incorporation of bromine into the benzene ring, i.e. o-TEMPO-Bz(Br)-C(O)-peptide, allows unambiguous distinction of the N-terminal peptide fragments from the C-terminal fragments through the unique bromine doublet isotopic signature. Furthermore, bromine substitution does not alter the overall radical-driven peptide backbone dissociation pathways of o-TEMPO-Bz-C(O)-peptide. From a practical perspective, the presence of the bromine isotopic signature in the N-terminal peptide fragments in TEMPO-assisted FRIPS MS represents a useful and cost-effective opportunity for de novo peptide sequencing. Copyright (c) 2015 John Wiley & Sons, Ltd. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | JOURNAL OF MASS SPECTROMETRY | - |
dc.subject | ELECTRON-CAPTURE DISSOCIATION | - |
dc.subject | GAS-PHASE FRAGMENTATION | - |
dc.subject | AMINO-ACID-SEQUENCES | - |
dc.subject | PROTEIN IDENTIFICATION | - |
dc.subject | CENTER-DOT | - |
dc.subject | TANDEM | - |
dc.subject | CATIONS | - |
dc.subject | CLEAVAGE | - |
dc.subject | DERIVATIZATION | - |
dc.subject | O-18 | - |
dc.title | Bromine isotopic signature facilitates de novo sequencing of peptides in free-radical-initiated peptide sequencing (FRIPS) mass spectrometry | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000351625400011 | - |
dc.identifier.doi | 10.1002/jms.3539 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MASS SPECTROMETRY, v.50, no.2, pp.378 - 387 | - |
dc.identifier.scopusid | 2-s2.0-84925345656 | - |
dc.citation.endPage | 387 | - |
dc.citation.startPage | 378 | - |
dc.citation.title | JOURNAL OF MASS SPECTROMETRY | - |
dc.citation.volume | 50 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Han, Sang Yun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | free-radical-initiated peptide sequencing (FRIPS) | - |
dc.subject.keywordAuthor | bromine signature | - |
dc.subject.keywordAuthor | isotopic pattern | - |
dc.subject.keywordAuthor | de novo sequencing | - |
dc.subject.keywordAuthor | radical-based tandem mass spectrometry | - |
dc.subject.keywordPlus | ELECTRON-CAPTURE DISSOCIATION | - |
dc.subject.keywordPlus | GAS-PHASE FRAGMENTATION | - |
dc.subject.keywordPlus | AMINO-ACID-SEQUENCES | - |
dc.subject.keywordPlus | PROTEIN IDENTIFICATION | - |
dc.subject.keywordPlus | CENTER-DOT | - |
dc.subject.keywordPlus | TANDEM | - |
dc.subject.keywordPlus | CATIONS | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | DERIVATIZATION | - |
dc.subject.keywordPlus | O-18 | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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