Intracellular Cl- as a signaling ion that potently regulates Na+/HCO3- transporters
DC Field | Value | Language |
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dc.contributor.author | Shcheynikov, Nikolay | - |
dc.contributor.author | Son, Aran | - |
dc.contributor.author | Hong, Jeong Hee | - |
dc.contributor.author | Yamazaki, Osamu | - |
dc.contributor.author | Ohana, Ehud | - |
dc.contributor.author | Kurtz, Ira | - |
dc.contributor.author | Shin, Dong Min | - |
dc.contributor.author | Muallem, Shmuel | - |
dc.date.available | 2020-02-28T10:44:01Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-01-20 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10863 | - |
dc.description.abstract | Cl- is a major anion in mammalian cells involved in transport processes that determines the intracellular activity of many ions and plasma membrane potential. Surprisingly, a role of intracellular Cl- (Cl-in(-)) as a signaling ion has not been previously evaluated. Here we report that Cl-in(-) functions as a regulator of cellular Na+ and HCO3- concentrations and transepithelial transport through modulating the activity of several electrogenic Na+-HCO3- transporters. We describe the molecular mechanism(s) of this regulation by physiological Cl-in(-) concentrations highlighting the role of GXXXP motifs in Cl-sensing. Regulation of the ubiquitous Na+-HCO3- co-transport (NBC)e1-B is mediated by two GXXXP-containing sites; regulation of NBCe2-C is dependent on a single GXXXP motif; and regulation of NBCe1-A depends on a cryptic GXXXP motif. In the basal state NBCe1-B is inhibited by high Cl-in(-) interacting at a low affinity GXXXP-containing site. IP3 receptor binding protein released with IP3 (IRBIT) activation of NBCe1-B unmasks a second high affinity Cl-in(-) interacting GXXXP-dependent site. By contrast, NBCe2-C, which does not interact with IRBIT, has a single high affinity N-terminal GXXP-containing Cl-in(-) interacting site. NBCe1-A is unaffected by Cl-in(-) between 5 and 140 mM. However, deletion of NBCe1-A residues 29-41 unmasks a cryptic GXXXP-containing site homologous with the NBCe1-B low affinity site that is involved in inhibition of NBCe1-A by Cl-in(-). These findings reveal a cellular Cl-in(-) sensing mechanism that plays an important role in the regulation of Na+ and HCO3- transport, with critical implications for the role of Cl-in(-) cellular ion homeostasis and epithelial fluid and electrolyte secretion. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.subject | RECEPTOR-BINDING PROTEIN | - |
dc.subject | FUNCTIONAL-CHARACTERIZATION | - |
dc.subject | CHLORIDE CHANNEL | - |
dc.subject | IP3 RECEPTOR | - |
dc.subject | SODIUM | - |
dc.subject | IRBIT | - |
dc.subject | NA+ | - |
dc.subject | FAMILY | - |
dc.subject | CFTR | - |
dc.subject | MODULATION | - |
dc.title | Intracellular Cl- as a signaling ion that potently regulates Na+/HCO3- transporters | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000348040700014 | - |
dc.identifier.doi | 10.1073/pnas.1415673112 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.112, no.3, pp.E329 - E337 | - |
dc.identifier.scopusid | 2-s2.0-84922283734 | - |
dc.citation.endPage | E337 | - |
dc.citation.startPage | E329 | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 112 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Hong, Jeong Hee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | intracellular Cl- | - |
dc.subject.keywordAuthor | signaling ion | - |
dc.subject.keywordAuthor | transporters | - |
dc.subject.keywordPlus | RECEPTOR-BINDING PROTEIN | - |
dc.subject.keywordPlus | FUNCTIONAL-CHARACTERIZATION | - |
dc.subject.keywordPlus | CHLORIDE CHANNEL | - |
dc.subject.keywordPlus | IP3 RECEPTOR | - |
dc.subject.keywordPlus | SODIUM | - |
dc.subject.keywordPlus | IRBIT | - |
dc.subject.keywordPlus | NA+ | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | CFTR | - |
dc.subject.keywordPlus | MODULATION | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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