Effects of coculture with cumulus-derived somatic cells on in vitro maturation of porcine oocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, Junchul David | - |
dc.contributor.author | Jeon, Yubyeol | - |
dc.contributor.author | Cai, Lian | - |
dc.contributor.author | Hwang, Seon-Ung | - |
dc.contributor.author | Kim, Eunhye | - |
dc.contributor.author | Lee, Eunsong | - |
dc.contributor.author | Kim, Dae Y. | - |
dc.contributor.author | Hyun, Sang-Hwan | - |
dc.date.available | 2020-02-28T10:44:02Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-01-15 | - |
dc.identifier.issn | 0093-691X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10865 | - |
dc.description.abstract | In the process of IVM, cumulus-oocyte complexes (COCs) separate from the follicular microenvironment, leading to the loss of endocrine interactions between follicular mural somatic cells and COCs. To restore the microenvironment, a coculture system was established using cumulus-derived somatic cells (CSCs) for IVM. The CSCs were cultured in Dulbecco's modified Eagle's medium for 48 hours with varying numbers of CSCs (0.0, 2.5 x 10(4), 5.0 x 10(4), and 10.0 x 10(4)) and then cultured in tissue culture medium 199 (TCM 199) for 4 hours before adding the oocytes. Cumulus-oocyte complexes from 3- to 6-mm follicles were matured in 500 mu L of TCM 199 with eCG and hCG for 22 hours and then cultured in TCM 199 without hormones for 22 hours. After IVM, the group with 2.5 x 10(4) CSCs showed a significant increase in intracellular glutathione levels compared with the control group. In the evaluation of sperm penetration, efficient fertilization was increased in the groups with 2.5 x 10(4) and 5.0 x 104 CSCs compared with controls (44.9 and 46.5 vs. 32.1, respectively). The mRNA expression pattern analysis in matured COCs showed a significant upregulation of PCNA, COX-2, Has2, Ptx3, and Nrf2 in the 2.5 x 10(4) CSC group compared with controls. During COC maturation at 0, 11, 22, 33, and 44 hours, the 2.5 x 10(4) and 5.0 x 10(4) CSC groups showed a significantly altered mRNA expression of BMP15 and GDF9. The developmental competence of the matured oocytes in all groups was evaluated after IVF and parthenogenetic activation (PA). After IVF, the 2.5 x 10(4) CSC group showed significantly higher cleavage, blastocyst formation rate, and total cell numbers compared with controls (60.0%, 35.7%, and 127.3 vs. 43.2%, 21.1%, and 89.3, respectively). After PA, the 2.5 x 10(4) CSC group had significantly higher blastocyst formation rate and total cell number than the control group (52.0% and 120.4 vs. 35.4% and 90.9, respectively). In conclusion, these results suggest that the presence of a population of 2.5 x 10(4) CSCs during IVM synergistically improved the developmental potential of 1VF- and PA-derived porcine embryos by increasing the intracellular glutathione level via changing of a specific gene expression pattern during oocyte maturation. (C) 2015 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.relation.isPartOf | THERIOGENOLOGY | - |
dc.subject | GROWTH-DIFFERENTIATION FACTOR-9 | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | EMBRYO DEVELOPMENT | - |
dc.subject | PIG OOCYTES | - |
dc.subject | DEVELOPMENTAL COMPETENCE | - |
dc.subject | OVARIAN-FOLLICLES | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | PREIMPLANTATION DEVELOPMENT | - |
dc.subject | INTRACELLULAR GLUTATHIONE | - |
dc.subject | GRANULOSA-CELLS | - |
dc.title | Effects of coculture with cumulus-derived somatic cells on in vitro maturation of porcine oocytes | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000346218000019 | - |
dc.identifier.doi | 10.1016/j.theriogenology.2014.09.025 | - |
dc.identifier.bibliographicCitation | THERIOGENOLOGY, v.83, no.2, pp.294 - 305 | - |
dc.identifier.scopusid | 2-s2.0-84919446799 | - |
dc.citation.endPage | 305 | - |
dc.citation.startPage | 294 | - |
dc.citation.title | THERIOGENOLOGY | - |
dc.citation.volume | 83 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Kim, Dae Y. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Porcine | - |
dc.subject.keywordAuthor | Oocyte | - |
dc.subject.keywordAuthor | In vitro maturation | - |
dc.subject.keywordAuthor | Coculture | - |
dc.subject.keywordAuthor | Somatic cell | - |
dc.subject.keywordPlus | GROWTH-DIFFERENTIATION FACTOR-9 | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | EMBRYO DEVELOPMENT | - |
dc.subject.keywordPlus | PIG OOCYTES | - |
dc.subject.keywordPlus | DEVELOPMENTAL COMPETENCE | - |
dc.subject.keywordPlus | OVARIAN-FOLLICLES | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | PREIMPLANTATION DEVELOPMENT | - |
dc.subject.keywordPlus | INTRACELLULAR GLUTATHIONE | - |
dc.subject.keywordPlus | GRANULOSA-CELLS | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.