Detection of DNAs by Using Dual Packed Polystyrene Bead-Quantum Dots in a Microfluidic Chip
- Authors
- Ngoc Tam Le; Kim, Jong Sung
- Issue Date
- Jan-2015
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Quantum Dots; Microfluidic Chip; DNA; p53 Gene Exon 6, 7
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.15, no.1, pp.100 - 104
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 15
- Number
- 1
- Start Page
- 100
- End Page
- 104
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10875
- DOI
- 10.1166/jnn.2015.8380
- ISSN
- 1533-4880
- Abstract
- The semiconductor nanocrystals (or quantum dots) have shown peculiar optical and electrical properties due to their exceptionally small size. In recent years, tremendous researches on quantum dots have been carried out. Among them, QDs as sensing media for biological assay have achieved a great progress. Recently we have reported the detection of DNAs by using fluorescence quenching of QDs after DNA hybridization. Several oligonucleotides and human genomic genes could be detected. In this report we used dual packing of polystyrene bead-quantum dots to detect different kinds of DNAs simultaneously. QDs with different emission peaks were used. Carboxylated-CdSe/ZnS QDs (emission: 525, 605 nm) could bind to microbeads of polystyrene/divinyl benzene via EDC/NHS cross-linking reaction. Polystyrene bead-QDs with different colors were packed in the channel of the microfluidic chip. The fluorescence quenching from the QDs by intercalating dye was observed after hybridization of exon 6 and 7 of p53 gene at the weir in the channel of microfluidic chip. The simultaneous fluorescence quenching of the QDs by PI and TOTO-3 were observed.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 공과대학 > 화공생명공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10875)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.