Amyloid-Beta-Activated Human Microglial Cells Through ER-Resident Proteins
DC Field | Value | Language |
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dc.contributor.author | Yoo, YongCheol | - |
dc.contributor.author | Byun, Kyunghee | - |
dc.contributor.author | Kang, Taewook | - |
dc.contributor.author | Bayarsaikhan, Delger | - |
dc.contributor.author | Kim, Jin Young | - |
dc.contributor.author | Oh, Seyeoun | - |
dc.contributor.author | Kim, Young Hye | - |
dc.contributor.author | Kim, Se-Young | - |
dc.contributor.author | Chung, Won-Il | - |
dc.contributor.author | Kim, Seung U. | - |
dc.contributor.author | Lee, Bonghee | - |
dc.contributor.author | Park, Young Mok | - |
dc.date.available | 2020-02-28T10:44:22Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10885 | - |
dc.description.abstract | Microglial activation in the central nervous system is a key event in the neuroinflammation that accompanies neurodegenerative diseases such as Alzheimers disease (AD). Among cytokines involved in microglial activation, amyloid beta (A beta) peptide is known to be a key molecule in the induction of diverse inflammatory products, which may lead to chronic inflammation in AD. However, proteomic studies of microglia in AD are limited due to lack of proper cell or animal model systems. In this study, we performed a proteomic analysis of A beta-stimulated human microglial cells using SILAC (stable isotope labeling with amino acids in cell culture) combined with LC-MS/MS. Results showed that 60 proteins increased or decreased their abundance by 1.5 fold or greater. Among these, ER-resident proteins such as SERPINH1, PDIA6, PDIA3, and PPIB were revealed to be key molecular biomarkers of human microglial activation by validation of the proteomic results by immunostaining, PCR, ELISA, and Western blot. Taken together, our data suggest that ER proteins play an essential role in human microglial activation by A beta and may be important molecular therapeutic targets for treatment of AD. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | JOURNAL OF PROTEOME RESEARCH | - |
dc.subject | QUANTITATIVE PROTEOMIC ANALYSIS | - |
dc.subject | ALZHEIMERS-DISEASE | - |
dc.subject | PROGRANULIN EXPRESSION | - |
dc.subject | MOLECULAR CHAPERONE | - |
dc.subject | PRECURSOR PROTEIN | - |
dc.subject | STRESS | - |
dc.subject | BRAIN | - |
dc.subject | NRF2 | - |
dc.subject | PATHOGENESIS | - |
dc.subject | CLEARANCE | - |
dc.title | Amyloid-Beta-Activated Human Microglial Cells Through ER-Resident Proteins | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000347506600019 | - |
dc.identifier.doi | 10.1021/pr500926r | - |
dc.identifier.bibliographicCitation | JOURNAL OF PROTEOME RESEARCH, v.14, no.1, pp.214 - 223 | - |
dc.identifier.scopusid | 2-s2.0-84920271140 | - |
dc.citation.endPage | 223 | - |
dc.citation.startPage | 214 | - |
dc.citation.title | JOURNAL OF PROTEOME RESEARCH | - |
dc.citation.volume | 14 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Byun, Kyunghee | - |
dc.contributor.affiliatedAuthor | Bayarsaikhan, Delger | - |
dc.contributor.affiliatedAuthor | Oh, Seyeoun | - |
dc.contributor.affiliatedAuthor | Lee, Bonghee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | amyloid beta | - |
dc.subject.keywordAuthor | human microglia | - |
dc.subject.keywordAuthor | tandem mass tags | - |
dc.subject.keywordAuthor | LC-MS/MS | - |
dc.subject.keywordPlus | QUANTITATIVE PROTEOMIC ANALYSIS | - |
dc.subject.keywordPlus | ALZHEIMERS-DISEASE | - |
dc.subject.keywordPlus | PROGRANULIN EXPRESSION | - |
dc.subject.keywordPlus | MOLECULAR CHAPERONE | - |
dc.subject.keywordPlus | PRECURSOR PROTEIN | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | NRF2 | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | CLEARANCE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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