One-step nucleic acid extraction and amplification on an integrated microdevice for early detection of cochlodinium polykrikoides
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nguyen, T.P.O. | - |
dc.contributor.author | Jung, S.W. | - |
dc.contributor.author | Trinh, K.T.L. | - |
dc.contributor.author | Tran, B.M. | - |
dc.contributor.author | Pham, Q.N. | - |
dc.contributor.author | Kim, J.-H. | - |
dc.contributor.author | Lee, N.Y. | - |
dc.date.available | 2020-02-28T10:46:07Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.issn | 0000-0000 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10996 | - |
dc.description.abstract | Herein, a monolithic polycarbonate (PC) microdevice was developed to handle both DNA extraction and polymerase chain reaction (PCR) processes successively. Target cell - Cochlodinium polykrikoides microalgae, the causative species for harmful algal blooms in South Korea - was firstly captured on amine-functionalized microbeads via electrostatic interaction and then lysed to release DNA into subsequent PCR channel. Gram-negative Escherichia coli bacterium was used to illustrate the adhesion phenomenon based on its similar negatively-charged surface in comparison with algal cells. A 244-bp amplicon from the genome of C. polykrikoides algae was successfully amplified using this microdevice, demonstrating its potential application on further on-site analysis for early algal detection. © 15CBMS-0001. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Chemical and Biological Microsystems Society | - |
dc.relation.isPartOf | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences | - |
dc.title | One-step nucleic acid extraction and amplification on an integrated microdevice for early detection of cochlodinium polykrikoides | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp.1274 - 1276 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84983271774 | - |
dc.citation.endPage | 1276 | - |
dc.citation.startPage | 1274 | - |
dc.citation.title | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences | - |
dc.contributor.affiliatedAuthor | Nguyen, T.P.O. | - |
dc.contributor.affiliatedAuthor | Trinh, K.T.L. | - |
dc.contributor.affiliatedAuthor | Tran, B.M. | - |
dc.contributor.affiliatedAuthor | Pham, Q.N. | - |
dc.contributor.affiliatedAuthor | Kim, J.-H. | - |
dc.contributor.affiliatedAuthor | Lee, N.Y. | - |
dc.type.docType | Conference Paper | - |
dc.subject.keywordAuthor | Amine-functionalized | - |
dc.subject.keywordAuthor | Cochlodinium polykrikoides | - |
dc.subject.keywordAuthor | Monolithic microdevice | - |
dc.subject.keywordPlus | Algae | - |
dc.subject.keywordPlus | Escherichia coli | - |
dc.subject.keywordPlus | Extraction | - |
dc.subject.keywordPlus | Nucleic acids | - |
dc.subject.keywordPlus | Adhesion phenomena | - |
dc.subject.keywordPlus | Cochlodinium polykrikoides | - |
dc.subject.keywordPlus | Escherichia coli bacteria | - |
dc.subject.keywordPlus | Functionalized | - |
dc.subject.keywordPlus | Harmful algal blooms | - |
dc.subject.keywordPlus | Micro-device | - |
dc.subject.keywordPlus | Negatively charged surfaces | - |
dc.subject.keywordPlus | On-site analysis | - |
dc.subject.keywordPlus | Polymerase chain reaction | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.