Active Molecular Chitosan (AMC-S1): Preparation and Characterization of Antibacterial Activity

Abstract

The aim of the present study is to prepare the chitosan hydrolysates and characterize their potential antibacterial activity. High molecular weight chitosan (CTSN-H) and medium molecular weight chitosan (MMWC, 2~10-kDa) were further degraded efficiently by using chitosanase from Bacillus subtilis JH-1122. Molecular weight (MW) of the final product was determined by MALDI-TOF MS, showed several peaks ranging from 0.8 to 2.5-kDa. Based on antibacterial activity of the hydrolysates, final products were named as active molecular chitosan (AMC-S1). AMC-S1 was evaluated as IC50 to 12.5 μg/mL of the concentration toward Candida albicans. Our present study demonstrates that AMC-S1 derived from the digestion of CTSN-H and MMWC can be selected as a potent factor affecting on antimicrobial activity against C. albicans for the industry and pharmaceutical applications.