Contribution of CNT1 and ENT1 to ribavirin uptake in human hepatocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최민구 | - |
dc.contributor.author | 김민혜 | - |
dc.contributor.author | 맹한주 | - |
dc.contributor.author | 송임숙 | - |
dc.date.available | 2020-02-28T13:45:00Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2015-05 | - |
dc.identifier.issn | 0253-6269 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/11591 | - |
dc.description.abstract | The objective of this study was to investigate thecontributions of a sodium-dependent concentrative nucleosidetransporter (CNT) 1 and an equilibrative nucleosidetransporter (ENT) 1 to ribavirin uptake in human hepatocytes. The initial studies in oocytes expressing CNT1 andENT1 showed increases in ribavirin uptake, indicating thatribavirin was a substrate for both CNT1 and ENT1. TheCNT1- and ENT1-mediated ribavirin uptake showed concentrationdependency with the following kinetics parameters:Km 26.3 lM and Vmax 426.2 fmol/min/oocyte forCNT1; Km 70.5 lM and Vmax 134.3 fmol/min/oocyte forENT1. Ribavirin uptake clearance in six human hepatocytesranged from 21.3 to 300.7 lL/min. Estimation of the contributionsof CNT1 and ENT1 to the hepatic uptake ofribavirin by using a relative activity factor method indicatedthat the relative contribution of ENT1 to the ribavirin uptakewas 82.8 ± 3.9 %. Real-time polymerase chain reactionanalysis of CNT1 and ENT1 expressions in the hepatocytesshowed that ENT1 mRNA expression was closely correlatedwith ribavirin uptake (R = 0.95, P = 0.003) while CNT1was not. The findings indicated that ENT1 was the majortransporter controlling the hepatic uptake of ribavirin. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 대한약학회 | - |
dc.relation.isPartOf | Archives of Pharmacal Research | - |
dc.title | Contribution of CNT1 and ENT1 to ribavirin uptake in human hepatocytes | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000354299800039 | - |
dc.identifier.doi | 10.1007/s12272-014-0437-y | - |
dc.identifier.bibliographicCitation | Archives of Pharmacal Research, v.38, no.5, pp.904 - 913 | - |
dc.identifier.kciid | ART001991037 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84939882650 | - |
dc.citation.endPage | 913 | - |
dc.citation.startPage | 904 | - |
dc.citation.title | Archives of Pharmacal Research | - |
dc.citation.volume | 38 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | 맹한주 | - |
dc.subject.keywordAuthor | Ribavirin | - |
dc.subject.keywordAuthor | Human hepatocytes | - |
dc.subject.keywordAuthor | CNT1 | - |
dc.subject.keywordAuthor | ENT1 | - |
dc.subject.keywordAuthor | mRNA expression | - |
dc.subject.keywordAuthor | Relative contribution | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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