Nanoclusters prepared from ruthenium(II) and quercetin for fluorometric detection of cobalt(II), and a method for screening their anticancer drug activity
DC Field | Value | Language |
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dc.contributor.author | Lakshmi, Buddolla Anantha | - |
dc.contributor.author | Bae, Jin-Young | - |
dc.contributor.author | An, Jeong Ho | - |
dc.contributor.author | Kim, Sanghyo | - |
dc.date.available | 2020-02-27T02:40:49Z | - |
dc.date.created | 2020-02-04 | - |
dc.date.issued | 2019-08 | - |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1160 | - |
dc.description.abstract | Ruthenium-quercetin conjugated nanoclusters (Ru-QC NCs) were synthesized via a one-pot reflux reaction. As inhalation of heavy metal ions like cobalt can lead to lung cancer, a fluorescent probe was designed for the determination of Co(II) both in aqueous solutions and living cells. The probe consists of hybrid nanoclusters with an average size of 2 nm that were prepared from ruthenium(II) ions and the flavonoid quercetin. These are termed as Ru-QC NCs. They display strong orange-colored emission with a peak at 558 nm under 465-nm excitation. The Ru-QC NCs are cell viable and enable imaging of cells and intracellular fluorometric detection of Co(II). The anticancer properties of Ru-QC NCs were screened by using non-small cell lung cancer (A549) and human dermal fibroblast (HDFa) cell lines. The Ru-QC NCs exert considerable cytotoxicity in A549 cells (at levels of 20-50 mu g.mL(-1)), whereas no significant cytotoxicity was observed in case of HDFa cells. The anticancer properties of Ru-QC NCs were screened via MTT assay, live-dead staining, and ROS assay, respectively. Morphological changes of cancer cells were observed using atomic force microscopy. The fluorescent probe can detect Co(II) with a detection limit of 9.28 nM and with a linear response in the 0.03-100 mu M concentration range. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER WIEN | - |
dc.relation.isPartOf | MICROCHIMICA ACTA | - |
dc.subject | FUNCTIONALIZED GOLD NANOPARTICLES | - |
dc.subject | METAL-ORGANIC FRAMEWORK | - |
dc.subject | COLORIMETRIC SENSORS | - |
dc.subject | COMPLEX | - |
dc.subject | CELLS | - |
dc.subject | PROBE | - |
dc.subject | DOTS | - |
dc.subject | ION | - |
dc.title | Nanoclusters prepared from ruthenium(II) and quercetin for fluorometric detection of cobalt(II), and a method for screening their anticancer drug activity | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000475964400008 | - |
dc.identifier.doi | 10.1007/s00604-019-3657-5 | - |
dc.identifier.bibliographicCitation | MICROCHIMICA ACTA, v.186, no.8 | - |
dc.identifier.scopusid | 2-s2.0-85068972231 | - |
dc.citation.title | MICROCHIMICA ACTA | - |
dc.citation.volume | 186 | - |
dc.citation.number | 8 | - |
dc.contributor.affiliatedAuthor | Lakshmi, Buddolla Anantha | - |
dc.contributor.affiliatedAuthor | Kim, Sanghyo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Atomic force microscopy | - |
dc.subject.keywordAuthor | Bioimaging | - |
dc.subject.keywordAuthor | Cytotoxicity | - |
dc.subject.keywordAuthor | Flavonoids | - |
dc.subject.keywordAuthor | Fluorescent probe | - |
dc.subject.keywordAuthor | Intracellular fluorosensing | - |
dc.subject.keywordAuthor | Live-dead staining | - |
dc.subject.keywordPlus | FUNCTIONALIZED GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | METAL-ORGANIC FRAMEWORK | - |
dc.subject.keywordPlus | COLORIMETRIC SENSORS | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | DOTS | - |
dc.subject.keywordPlus | ION | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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