급성 아토피 피부염 마우스 모델에서 한약조합약물의 염증반응 진정효과

Abstract

Object : The object of this study was effected of composed of 4 herbal medicines(Rubus coreanus, RehmanniaeRadix, Houttuynia cordata, Betulae cortex) on acute atopic dermatitis mice. Methods : BALB/c mice were divided 4 groups ; normal group, negative control group of acute atopic dermatitismice induced by DNCB, treated apply to the back skin with the herbal medicine group, and treated orally withherbal medicine group. Treated with herbal medicine and DNCB during 2 weeks. After treated, measured WBCs,RBCs, and platelet in the blood, and analysed mRNA expression of spleen. Result : Composed herbal medicines could reduce WBCs, lymphocytes, monocytes, neutrophils, and eosinophilsin the mouse blood. And, could down TNF-α levels in spleen. In the skin tissue histology results, herbalmedicines reduce the cells and T cells. Conclusion : Composed herbal medicines help to another inflammatory disease because they reduce the cellsand T cells in the skin tissues. And they have inhibitory effects of TNF-α mRNA expression, and Th 2 immuneresponses. Therefore herbal medicines use for an acute atopic dermatitis treatment.