遠志물추출물이 Apoptosis에 미치는 효과
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이상철 | - |
dc.contributor.author | 김연섭 | - |
dc.date.available | 2020-02-28T14:43:15Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1229-1765 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/11763 | - |
dc.description.abstract | Objectives : The purpose of this study was to observe the effects of Polygalae Radix(PR) on 4-HNE-induced apoptosis in PC-12 cell. Methods : A MTT assay was conducted to observe the cytotoxicity of Polygalae Radix on the cell viability and the cytoprotective effect of Polygalae Radix against 4-HNE that causes oxidative stress-induced cytotoxicity, and then a western blot was conducted to observe the expression of TNF-α, caspase-3, Bax and Bcl-2 protein that are important factors involved with apoptosis signaling pathway. Results : The Polygalae Radix water extract 25 ㎍, 50 ㎍, 100 ㎍ and 200 ㎍/mL had no cytotoxicity on the PC-12 cell. The Polygalae Radix water extract 25 ㎍, 50 ㎍ and 100 ㎍/mL had the cytoprotective effect against 4-HNE that causes cytotoxicity on the PC-12 cell. The Polygalae Radix water extract 50 ㎍/mL significantly suppressed the increase in TNF-α protein expression in PC-12 cell. The Polygalae Radix water extract 25 ㎍ and 50 ㎍/mL significantly suppressed the increase in caspase-3 protein expression in PC-12 cell. The Polygalae Radix water extract 25 ㎍, 50 ㎍ and 100 ㎍/mL suppressed the increase in Bax protein expression in PC-12 cell but had no significance. The Polygalae Radix water extract 25 ㎍ and 100 ㎍/mL significantly prevented the decrease in Bcl-2 protein expression in PC-12 cell,Conclusions : These results suggest that the Polygalae Radix water extract is effective in inhibiting apoptosis. | - |
dc.language | 한국어 | - |
dc.language.iso | ko | - |
dc.publisher | 대한본초학회 | - |
dc.relation.isPartOf | 대한본초학회지 | - |
dc.title | 遠志물추출물이 Apoptosis에 미치는 효과 | - |
dc.title.alternative | Effects of Polygalae Radix on Apotosis in PC-12 Cell | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | 대한본초학회지, v.30, no.1, pp.59 - 65 | - |
dc.identifier.kciid | ART001960183 | - |
dc.citation.endPage | 65 | - |
dc.citation.startPage | 59 | - |
dc.citation.title | 대한본초학회지 | - |
dc.citation.volume | 30 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | 이상철 | - |
dc.contributor.affiliatedAuthor | 김연섭 | - |
dc.subject.keywordAuthor | Polygalae Radix | - |
dc.subject.keywordAuthor | 4-HNE | - |
dc.subject.keywordAuthor | TNF-α | - |
dc.subject.keywordAuthor | caspase-3 | - |
dc.subject.keywordAuthor | Bax | - |
dc.subject.keywordAuthor | Bcl-2 | - |
dc.description.journalRegisteredClass | kci | - |
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