Investigation of cellular responses upon interaction with silver nanoparticles
DC Field | Value | Language |
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dc.contributor.author | Subbiah, Ramesh | - |
dc.contributor.author | Jeon, Seong Beom | - |
dc.contributor.author | Park, Kwideok | - |
dc.contributor.author | Ahn, Sang Jung | - |
dc.contributor.author | Yun, Kyusik | - |
dc.date.available | 2020-02-28T15:41:58Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1178-2013 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/11969 | - |
dc.description.abstract | In order for nanoparticles (NPs) to be applied in the biomedical field, a thorough investigation of their interactions with biological systems is required. Although this is a growing area of research, there is a paucity of comprehensive data in cell-based studies. To address this, we analyzed the physicomechanical responses of human alveolar epithelial cells (A549), mouse fibroblasts (NIH3T3), and human bone marrow stromal cells (HS-5), following their interaction with silver nanoparticles (AgNPs). When compared with kanamycin, AgNPs exhibited moderate antibacterial activity. Cell viability ranged from <80% at a high AgNPs dose (40 mu g/mL) to.95% at a low dose (10 mu g/mL). We also used atomic force microscopy-coupled force spectroscopy to evaluate the biophysical and biomechanical properties of cells. This revealed that AgNPs treatment increased the surface roughness (P < 0.001) and stiffness (P<0.001) of cells. Certain cellular changes are likely due to interaction of the AgNPs with the cell surface. The degree to which cellular morphology was altered directly proportional to the level of AgNP-induced cytotoxicity. Together, these data suggest that atomic force microscopy can be used as a potential tool to develop a biomechanics-based biomarker for the evaluation of NP-dependent cytotoxicity and cytopathology. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | DOVE MEDICAL PRESS LTD | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF NANOMEDICINE | - |
dc.subject | ATOMIC-FORCE MICROSCOPY | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | CELLS | - |
dc.title | Investigation of cellular responses upon interaction with silver nanoparticles | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000365122300020 | - |
dc.identifier.doi | 10.2147/IJN.S88508 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF NANOMEDICINE, v.10, pp.191 - 201 | - |
dc.identifier.scopusid | 2-s2.0-84947443063 | - |
dc.citation.endPage | 201 | - |
dc.citation.startPage | 191 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF NANOMEDICINE | - |
dc.citation.volume | 10 | - |
dc.contributor.affiliatedAuthor | Yun, Kyusik | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | AFM | - |
dc.subject.keywordAuthor | roughness | - |
dc.subject.keywordAuthor | nanoindentation | - |
dc.subject.keywordAuthor | biomarker | - |
dc.subject.keywordAuthor | cytotoxicity | - |
dc.subject.keywordAuthor | biomechanics | - |
dc.subject.keywordPlus | ATOMIC-FORCE MICROSCOPY | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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