miR-543 and miR-590-3p regulate human mesenchymal stem cell aging via direct targeting of AIMP3/p18
DC Field | Value | Language |
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dc.contributor.author | Lee, Seunghee | - |
dc.contributor.author | Yu, Kyung-Rok | - |
dc.contributor.author | Ryu, Young-Sil | - |
dc.contributor.author | Oh, Young Sun | - |
dc.contributor.author | Hong, In-Sun | - |
dc.contributor.author | Kim, Hyung-Sik | - |
dc.contributor.author | Lee, Jin Young | - |
dc.contributor.author | Kim, Sunghoon | - |
dc.contributor.author | Seo, Kwang-Won | - |
dc.contributor.author | Kang, Kyung-Sun | - |
dc.date.available | 2020-02-28T15:43:34Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2014-12 | - |
dc.identifier.issn | 0161-9152 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12058 | - |
dc.description.abstract | Previously, AIMP3 (aminoacyl-tRNAsynthetase- interacting multifunctional protein-3) was shown to be involved in the macromolecular tRNA synthetase complex or to act as a tumor suppressor. In this study, we report a novel role of AIMP3/p18 in the cellular aging of human mesenchymal stem cells (hMSCs). We found that AIMP3/p18 expression significantly increased in senescent hMSCs and in aged mouse bone marrow-derived MSCs (mBM-MSCs). AIMP3/p18 overexpression is sufficient to induce the cellular senescence phenotypes with compromised clonogenicity and adipogenic differentiation potential. To identify the upstream regulators of AIMP3/p18 during senescence, we screened for potential epigenetic regulators and for miRNAs. We found that the levels of miR-543 and miR-590-3p significantly decreased under senescence-inducing conditions, whereas the AIMP3/p18 protein levels increased. We demonstrate for the first time that miR-543 and miR-590-3p are able to decrease AIMP3/p18 expression levels through direct binding to the AIMP/p18 transcripts, which further compromised the induction of the senescence phenotype. Taken together, our data demonstrate that AIMP3/p18 regulates cellular aging in hMSCs possibly through miR-543 and miR-590-3p. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.relation.isPartOf | AGE | - |
dc.subject | REPLICATIVE SENESCENCE | - |
dc.subject | TRANSFER-RNA | - |
dc.subject | MICRORNA | - |
dc.subject | PROLIFERATION | - |
dc.subject | P53 | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | TRANSLATION | - |
dc.subject | INHIBITORS | - |
dc.subject | PHENOTYPE | - |
dc.subject | APOPTOSIS | - |
dc.title | miR-543 and miR-590-3p regulate human mesenchymal stem cell aging via direct targeting of AIMP3/p18 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000346770900006 | - |
dc.identifier.doi | 10.1007/s11357-014-9724-2 | - |
dc.identifier.bibliographicCitation | AGE, v.36, no.6 | - |
dc.identifier.scopusid | 2-s2.0-84919807883 | - |
dc.citation.title | AGE | - |
dc.citation.volume | 36 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Hong, In-Sun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | AIMP3/p18 | - |
dc.subject.keywordAuthor | Stemcell | - |
dc.subject.keywordAuthor | Aging | - |
dc.subject.keywordAuthor | miR-543 | - |
dc.subject.keywordAuthor | miR-590-3p | - |
dc.subject.keywordPlus | REPLICATIVE SENESCENCE | - |
dc.subject.keywordPlus | TRANSFER-RNA | - |
dc.subject.keywordPlus | MICRORNA | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | PHENOTYPE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.relation.journalResearchArea | Geriatrics & Gerontology | - |
dc.relation.journalWebOfScienceCategory | Geriatrics & Gerontology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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