Induction of Caspase-dependent Apoptosis by Apigenin by Inhibiting STAT3 Signaling in HER2-overexpressing MDA-MB-453 Breast Cancer Cells
DC Field | Value | Language |
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dc.contributor.author | Seo, Hye-Sook | - |
dc.contributor.author | Ku, Jin Mo | - |
dc.contributor.author | Choi, Han-Seok | - |
dc.contributor.author | Woo, Jong-Kyu | - |
dc.contributor.author | Jang, Bo-Hyoung | - |
dc.contributor.author | Shin, Yong Cheol | - |
dc.contributor.author | Ko, Seong-Gyu | - |
dc.date.available | 2020-02-28T17:42:38Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.issn | 0250-7005 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12591 | - |
dc.description.abstract | Background: This study aimed to examine the effect of apigenin on proliferation and apoptosis in HER2-overexpressing MDA-MB-453 breast cancer cells. Materials and Methods: The antiproliferative effects of apigenin were examined by proliferation and MTT assays. The effect of apigenin on apoptotic molecules was determined by western blotting. RT-PCR was performed to measure mRNA levels of HIF-1 alpha and VEGF. ELISA assay was performed to measure intracellular VEGF levels. Immunocytochemistry was performed to evaluate nuclear STAT3 level. Results: Apigenin inhibited the proliferation of MDA-MB-453 cells. Apigenin up-regulated the levels of cleaved caspase-8 and caspase-3, and induced the cleavage of PARP. Apigenin induced extrinsic apoptosis and blocked the activation (phosphorylation) of JAK2 and STAT3. Apigenin inhibited CoCl2-induced VEGF secretion and decreased the nuclear staining of STAT3. Conclusion: Apigenin exerts its antiproliferative activity by inhibiting STAT3 signaling. Apigenin could serve as a useful compound to prevent or treat HER2-overexpressing breast cancer. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | INT INST ANTICANCER RESEARCH | - |
dc.relation.isPartOf | ANTICANCER RESEARCH | - |
dc.subject | FACTOR-KAPPA-B | - |
dc.subject | CYCLE ARREST | - |
dc.subject | VEGF EXPRESSION | - |
dc.subject | ACTIVATION | - |
dc.subject | RECEPTOR | - |
dc.subject | PATHWAY | - |
dc.subject | DEATH | - |
dc.subject | MELANOMA | - |
dc.subject | PROSTATE | - |
dc.subject | LINES | - |
dc.title | Induction of Caspase-dependent Apoptosis by Apigenin by Inhibiting STAT3 Signaling in HER2-overexpressing MDA-MB-453 Breast Cancer Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000336875200022 | - |
dc.identifier.bibliographicCitation | ANTICANCER RESEARCH, v.34, no.6, pp.2869 - 2882 | - |
dc.identifier.scopusid | 2-s2.0-84906314590 | - |
dc.citation.endPage | 2882 | - |
dc.citation.startPage | 2869 | - |
dc.citation.title | ANTICANCER RESEARCH | - |
dc.citation.volume | 34 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Woo, Jong-Kyu | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Breast cancer | - |
dc.subject.keywordAuthor | HER2 | - |
dc.subject.keywordAuthor | apigenin | - |
dc.subject.keywordAuthor | apotosis | - |
dc.subject.keywordAuthor | STAT3 | - |
dc.subject.keywordAuthor | VEGF | - |
dc.subject.keywordPlus | FACTOR-KAPPA-B | - |
dc.subject.keywordPlus | CYCLE ARREST | - |
dc.subject.keywordPlus | VEGF EXPRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | MELANOMA | - |
dc.subject.keywordPlus | PROSTATE | - |
dc.subject.keywordPlus | LINES | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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