Characterization of an alkalophilic extracellular chitosanase from Bacillus cereus GU-02
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Goo, Bon Geun | - |
dc.contributor.author | Park, Jae Kweon | - |
dc.date.available | 2020-02-28T17:42:55Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.issn | 1389-1723 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12604 | - |
dc.description.abstract | An alkalophilic extracellular chitosanase (ACTase) was characterized from the culture supernatant of Bacillus cereus GU-02. Kinetic properties of ACTase produced from B. cereus GU-02 after cultivation in anaerobic condition, alkaline medium (pH 10) at 37 degrees C for 3 days were investigated. ACTase was found to be stable in alkaline pH range from 8 to 10. Interestingly, optimum pH and temperature were estimated to be 10 and 37 degrees C, respectively, where ACTase showed chitosan degrading activity (87%), which was enhanced by 15% in the presence of calcium ions (8 mM). The ACTase produced from B. cereus GU-02 was partially purified from the culture supernatant, and its enzymatic activity was kinetically characterized. The V-max and K-m were estimated with a chitosan (degree of deacetylation, DD 92% as substrate) as 0.038 U/min/mu g protein and 0327 mu M, respectively. A combination of the TIC and MALDI-TOF MS results showed that the chitosan oligosaccharides obtained from the hydrolysis of high molecular weight chitosan (HMWC) by ACTase of the B. cereus GU-2 comprise oligomers with degree of polymerization (DP) mainly from dimers to pentamers. High production of ACTase and chitooligosaccharides may be useful for various industrial and biological applications. (C) 2013, The Society for Biotechnology, Japan. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SOC BIOSCIENCE BIOENGINEERING JAPAN | - |
dc.relation.isPartOf | JOURNAL OF BIOSCIENCE AND BIOENGINEERING | - |
dc.subject | HEXA-N-ACETYLCHITOHEXAOSE | - |
dc.subject | PURIFICATION | - |
dc.subject | CHITIN | - |
dc.subject | OLIGOSACCHARIDES | - |
dc.subject | SPECIFICITY | - |
dc.subject | MODE | - |
dc.title | Characterization of an alkalophilic extracellular chitosanase from Bacillus cereus GU-02 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000339219400006 | - |
dc.identifier.doi | 10.1016/j.jbiosc.2013.11.005 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v.117, no.6, pp.684 - 689 | - |
dc.identifier.scopusid | 2-s2.0-84899650748 | - |
dc.citation.endPage | 689 | - |
dc.citation.startPage | 684 | - |
dc.citation.title | JOURNAL OF BIOSCIENCE AND BIOENGINEERING | - |
dc.citation.volume | 117 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Goo, Bon Geun | - |
dc.contributor.affiliatedAuthor | Park, Jae Kweon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Bacillus cereus | - |
dc.subject.keywordAuthor | Alkalophilic chitosanase | - |
dc.subject.keywordAuthor | Chitosan | - |
dc.subject.keywordAuthor | Chitooligosaccharides | - |
dc.subject.keywordAuthor | Kinetic properties | - |
dc.subject.keywordPlus | HEXA-N-ACETYLCHITOHEXAOSE | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | CHITIN | - |
dc.subject.keywordPlus | OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | MODE | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.