The anti-inflammatory potential of Cortex Phellodendron in vivo and in vitro: Down-regulation of NO and iNOS through suppression of NF-kappa B and MAPK activation
DC Field | Value | Language |
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dc.contributor.author | Choi, You Yeon | - |
dc.contributor.author | Kim, Mi Hye | - |
dc.contributor.author | Han, Jae Min | - |
dc.contributor.author | Hong, Jongki | - |
dc.contributor.author | Lee, Tae-Hee | - |
dc.contributor.author | Kim, Sung-Hoon | - |
dc.contributor.author | Yang, Woong Mo | - |
dc.date.available | 2020-02-28T17:45:57Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2014-04 | - |
dc.identifier.issn | 1567-5769 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12748 | - |
dc.description.abstract | Cortex Phellodendri amurensis (CPA), derived from the dried bark of Phellodendron amurense Rupr., is a traditional medicine widely used to treat various inflammation-related diseases. The aim of this study was to investigate the anti-inflammatory activity and molecular mechanism of CPA in vivo and in vitro. Mice were pretreated with CPA (200 mg/kg, p.o.) for three consecutive days; 2 h after the last CPA treatment, mice were intraperitoneally injected with lipopolysaccharide (LPS) to induce endotoxemia (35 mg/kg). After treatment, we assessed survival rate, protein levels and cytokine expression. In addition, we confirmed the molecular mechanism of anti-inflammatory effects of CPA in LPS-stimulated macrophage RAW 264.7 cells. The results showed that CPA significantly increased mice survival rates and down-regulated LPS-induced interleukin (IL)-6, IL-1 beta and macrophage chemo-attractant protein (MCP)-1 in serum. In addition, CPA inhibited inducible nitric oxide synthase (iNOS), activation of nuclear factor (NF)-kappa B by degradation and phosphorylation of I kappa B alpha, and attenuated phosphorylation of mitogen-activated protein kinases (MAPKs; ERIC 1/2, p38 and JNK) from mice challenged with LPS. Moreover, in RAW 264.7 cells, CPA dose-dependently down-regulated LPS-stimulated NO, iNOS expression, as well as inflammatory cytokines and protein expression, consistent with the results in vivo. The anti-inflammatory properties of CPA in vitro and in vivo suggest its utility for attenuating inflammation-related diseases. (C) 2014 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | INTERNATIONAL IMMUNOPHARMACOLOGY | - |
dc.subject | NITRIC-OXIDE SYNTHASE | - |
dc.subject | MACROPHAGE ACTIVATION | - |
dc.subject | EXPRESSION | - |
dc.subject | CELLS | - |
dc.subject | LIPOPOLYSACCHARIDE | - |
dc.subject | PHYTOCHEMICALS | - |
dc.subject | AMURENSE | - |
dc.subject | PATHWAY | - |
dc.subject | COX-2 | - |
dc.subject | ACID | - |
dc.title | The anti-inflammatory potential of Cortex Phellodendron in vivo and in vitro: Down-regulation of NO and iNOS through suppression of NF-kappa B and MAPK activation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000334133600004 | - |
dc.identifier.doi | 10.1016/j.intimp.2014.01.020 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL IMMUNOPHARMACOLOGY, v.19, no.2, pp.214 - 220 | - |
dc.identifier.scopusid | 2-s2.0-84896707179 | - |
dc.citation.endPage | 220 | - |
dc.citation.startPage | 214 | - |
dc.citation.title | INTERNATIONAL IMMUNOPHARMACOLOGY | - |
dc.citation.volume | 19 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Lee, Tae-Hee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Phellodendron amurense | - |
dc.subject.keywordAuthor | Lipopolysaccharide (LPS) | - |
dc.subject.keywordAuthor | Cytokine | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | MACROPHAGE ACTIVATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | PHYTOCHEMICALS | - |
dc.subject.keywordPlus | AMURENSE | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | COX-2 | - |
dc.subject.keywordPlus | ACID | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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