A Conserved Mechanism for Binding of p53 DNA-Binding Domain and Anti-Apoptotic Bcl-2 Family Proteins
DC Field | Value | Language |
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dc.contributor.author | Lee, Dong-Hwa | - |
dc.contributor.author | Ha, Ji-Hyang | - |
dc.contributor.author | Kim, Yul | - |
dc.contributor.author | Jang, Mi | - |
dc.contributor.author | Park, Sung Jean | - |
dc.contributor.author | Yoon, Ho Sup | - |
dc.contributor.author | Kim, Eun-Hee | - |
dc.contributor.author | Bae, Kwang-Hee | - |
dc.contributor.author | Park, Byoung Chul | - |
dc.contributor.author | Park, Sung Goo | - |
dc.contributor.author | Yi, Gwan-Su | - |
dc.contributor.author | Chi, Seung-Wook | - |
dc.date.available | 2020-02-28T17:46:29Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2014-03-31 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12770 | - |
dc.description.abstract | The molecular interaction between tumor suppressor p53 and the anti-apoptotic Bcl-2 family proteins plays an essential role in the transcription-independent apoptotic pathway of p53. In this study, we investigated the binding of p53 DNA-binding domain (p53DBD) with the anti-apoptotic Bcl-2 family proteins, Bcl-w, Mcl-1, and Bcl-2, using GST pull-down assay and NMR spectroscopy. The GST pull-down assays and NMR experiments demonstrated the direct binding of the p53DBD with Bcl-w, Mcl-1, and Bcl-2. Further, NMR chemical shift perturbation data showed that Bcl-w and Mcl-1 bind to the positively charged DNA-binding surface of p53DBD. Noticeably, the refined structural models of the complexes between p53DBD and Bcl-w, Mcl-1, and Bcl-2 showed that the binding mode of p53DBD is highly conserved among the anti-apoptotic Bcl-2 family proteins. Furthermore, the chemical shift perturbations on Bcl-w, Mcl-1, and Bcl-2 induced by p53DBD binding occurred not only at the p53DBD-binding acidic region but also at the BH3 peptide-binding pocket, which suggests an allosteric conformational change similar to that observed in Bcl-XL. Taken altogether, our results revealed a structural basis for a conserved binding mechanism between p53DBD and the anti-apoptotic Bcl-2 family proteins, which shed light on to the molecular understanding of the transcription-independent apoptosis pathway of p53. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.subject | STRUCTURAL INSIGHTS | - |
dc.subject | MUTANTS | - |
dc.subject | NUCLEAR | - |
dc.subject | MCL-1 | - |
dc.subject | SITE | - |
dc.subject | BAK | - |
dc.title | A Conserved Mechanism for Binding of p53 DNA-Binding Domain and Anti-Apoptotic Bcl-2 Family Proteins | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000342559800011 | - |
dc.identifier.doi | 10.14348/molcells.2014.0001 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.37, no.3, pp.264 - 269 | - |
dc.identifier.kciid | ART001859977 | - |
dc.identifier.scopusid | 2-s2.0-84896923814 | - |
dc.citation.endPage | 269 | - |
dc.citation.startPage | 264 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 37 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Park, Sung Jean | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | Bcl-2 family proteins | - |
dc.subject.keywordAuthor | binding mechanism | - |
dc.subject.keywordAuthor | DNA-binding domain | - |
dc.subject.keywordAuthor | p53 | - |
dc.subject.keywordPlus | STRUCTURAL INSIGHTS | - |
dc.subject.keywordPlus | MUTANTS | - |
dc.subject.keywordPlus | NUCLEAR | - |
dc.subject.keywordPlus | MCL-1 | - |
dc.subject.keywordPlus | SITE | - |
dc.subject.keywordPlus | BAK | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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