Bacillus thuringiensis: a specific gamma-cyclodextrin producer strain
- Authors
- Goo, Bon Geun; Hwang, You Jin; Park, Jae Kweon
- Issue Date
- 11-Mar-2014
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Bacillus thuringiensis; Cyclodextrin; Cyclodextrin glycotransferase (CGTase); gamma-CD
- Citation
- CARBOHYDRATE RESEARCH, v.386, pp.12 - 17
- Journal Title
- CARBOHYDRATE RESEARCH
- Volume
- 386
- Start Page
- 12
- End Page
- 17
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12785
- DOI
- 10.1016/j.carres.2013.12.005
- ISSN
- 0008-6215
- Abstract
- An anaerobic microbial isolate Bacillus species, designated B. thuringiensis GU-2, was isolated from soil as a specific gamma-cyclodextrin (CD) producer strain in alkaline medium under anaerobic conditions. The optimum pH and temperature for bacterial growth and gamma-CD production were estimated to be pH 8.5 and 37 degrees C in the presence of 1.0% starch substrate, respectively. A high purity yield > 95% of gamma-CD from the total CD yield in the reaction mixture was obtained from starch that was supposed to be converted by gamma-cyclodextrin glycotransferase, tentatively named as gamma-CGTase. The maximum gamma-CGTase activity was estimated at 2.45 U/mL under optimized condition. This is the first report demonstrating the generation of a specific c-cyclodextrin (CD) producer strain by the action of a gamma-CGTase under anaerobic conditions. (C) 2014 Elsevier Ltd. All rights reserved.
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