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Cited 8 time in webofscience Cited 13 time in scopus
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Bacillus thuringiensis: a specific gamma-cyclodextrin producer strain

Authors
Goo, Bon GeunHwang, You JinPark, Jae Kweon
Issue Date
11-Mar-2014
Publisher
ELSEVIER SCI LTD
Keywords
Bacillus thuringiensis; Cyclodextrin; Cyclodextrin glycotransferase (CGTase); gamma-CD
Citation
CARBOHYDRATE RESEARCH, v.386, pp.12 - 17
Journal Title
CARBOHYDRATE RESEARCH
Volume
386
Start Page
12
End Page
17
URI
https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12785
DOI
10.1016/j.carres.2013.12.005
ISSN
0008-6215
Abstract
An anaerobic microbial isolate Bacillus species, designated B. thuringiensis GU-2, was isolated from soil as a specific gamma-cyclodextrin (CD) producer strain in alkaline medium under anaerobic conditions. The optimum pH and temperature for bacterial growth and gamma-CD production were estimated to be pH 8.5 and 37 degrees C in the presence of 1.0% starch substrate, respectively. A high purity yield > 95% of gamma-CD from the total CD yield in the reaction mixture was obtained from starch that was supposed to be converted by gamma-cyclodextrin glycotransferase, tentatively named as gamma-CGTase. The maximum gamma-CGTase activity was estimated at 2.45 U/mL under optimized condition. This is the first report demonstrating the generation of a specific c-cyclodextrin (CD) producer strain by the action of a gamma-CGTase under anaerobic conditions. (C) 2014 Elsevier Ltd. All rights reserved.
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바이오나노대학 > 생명과학과 > 1. Journal Articles
보건과학대학 > 의용생체공학과 > 1. Journal Articles

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