Co-culture with NK-92MI cells enhanced the anti-cancer effect of bee venom on NSCLC cells by inactivation of NF-kappa B
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kollipara, Pushpa Saranya | - |
dc.contributor.author | Kim, Jung Hyun | - |
dc.contributor.author | Won, Dohee | - |
dc.contributor.author | Lee, Sang Min | - |
dc.contributor.author | Sung, Ha Chang | - |
dc.contributor.author | Chang, Hyun Sok | - |
dc.contributor.author | Lee, Kang Tae | - |
dc.contributor.author | Lee, Kang Sik | - |
dc.contributor.author | Park, Mi Hee | - |
dc.contributor.author | Song, Min Jong | - |
dc.contributor.author | Song, Ho Sueb | - |
dc.contributor.author | Hong, Jin Tae | - |
dc.date.available | 2020-02-28T17:46:51Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2014-03 | - |
dc.identifier.issn | 0253-6269 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12793 | - |
dc.description.abstract | In the present study we experimented on a multimodal therapeutic approach, such as combining chemotherapy agent (Bee venom) with cellular (NK-92MI) immunotherapy. Previously bee venom has been found to show anti-cancer effect in various cancer cell lines. In lung cancer cells bee venom showed an IC50 value of 3 mu g/ml in both cell lines. The co-culture of NK-92MI cell lines with lung cancer cells also show a decrease in viability upto 50 % at 48 h time point. Hence we used bee venom treated NK-92MI cells to co-culture with NSCLC cells and found that there is a further decrease in cell viability upto 70 and 75 % in A549 and NCI-H460 cell lines respectively. We further investigated the expression of various apoptotic and anti-apoptotic proteins and found that Bax, cleaved caspase-3 and -8 were increasing where as Bcl-2 and cIAP-2 was decreasing. The expression of various death receptor proteins like DR3, DR6 and Fas was also increasing. Concomitantly the expression of various death receptor ligands (TNFalpha, Apo3L and FasL) was also increasing of NK-92MI cells after co-culture. Further the DNA binding activity and luciferase activity of NF-kappa B was also inhibited after co-culture with bee venom treated NK-92MI cell lines. The knock down of death receptors with si-RNA has reversed the decrease in cell viability and NF-kappa B activity after co-culture with bee venom treated NK-92MI cells. Thus this new approach can enhance the anti-cancer effect of bee venom at a much lower concentration. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PHARMACEUTICAL SOC KOREA | - |
dc.relation.isPartOf | ARCHIVES OF PHARMACAL RESEARCH | - |
dc.subject | NATURAL-KILLER-CELLS | - |
dc.subject | CANCER-CELLS | - |
dc.subject | LUNG-CANCER | - |
dc.subject | DEATH RECEPTORS | - |
dc.subject | MEDIATED LYSIS | - |
dc.subject | TUMOR-CELLS | - |
dc.subject | APOPTOSIS | - |
dc.subject | ACTIVATION | - |
dc.subject | INHIBITION | - |
dc.subject | INDUCTION | - |
dc.title | Co-culture with NK-92MI cells enhanced the anti-cancer effect of bee venom on NSCLC cells by inactivation of NF-kappa B | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000332480600011 | - |
dc.identifier.doi | 10.1007/s12272-013-0319-8 | - |
dc.identifier.bibliographicCitation | ARCHIVES OF PHARMACAL RESEARCH, v.37, no.3, pp.379 - 389 | - |
dc.identifier.kciid | ART001902199 | - |
dc.identifier.scopusid | 2-s2.0-84896393836 | - |
dc.citation.endPage | 389 | - |
dc.citation.startPage | 379 | - |
dc.citation.title | ARCHIVES OF PHARMACAL RESEARCH | - |
dc.citation.volume | 37 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Kim, Jung Hyun | - |
dc.contributor.affiliatedAuthor | Song, Ho Sueb | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | NK-92MI cells | - |
dc.subject.keywordAuthor | Lung cancer | - |
dc.subject.keywordAuthor | Bee venom | - |
dc.subject.keywordAuthor | Death receptor | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordPlus | NATURAL-KILLER-CELLS | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | LUNG-CANCER | - |
dc.subject.keywordPlus | DEATH RECEPTORS | - |
dc.subject.keywordPlus | MEDIATED LYSIS | - |
dc.subject.keywordPlus | TUMOR-CELLS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.