Eucommia ulmoides Oliv. bark. attenuates 6-hydroxydopamine-induced neuronal cell death through inhibition of oxidative stress in SH-SY5Y cells
DC Field | Value | Language |
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dc.contributor.author | Kwon, Seung-Hwan | - |
dc.contributor.author | Ma, Shi-Xun | - |
dc.contributor.author | Hong, Sa-Ik | - |
dc.contributor.author | Kim, Sun Yeou | - |
dc.contributor.author | Lee, Seok-Yong | - |
dc.contributor.author | Jang, Choon-Gon | - |
dc.date.available | 2020-02-28T18:41:43Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2014-02-27 | - |
dc.identifier.issn | 0378-8741 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12835 | - |
dc.description.abstract | Ethno pharmacological relevance: Eucommia ulmoides Oliv. Bark. (EUE) has commonly been used to fortify the muscles and lungs, lower blood pressure, prevent miscarriage, improve liver and kidney tone, and promote longevity as a traditional tonic medicine in Korea, China, and Japan. Aim of the study: In this study, we investigated the mechanisms by which EUE protects neuronal cells from apoptosis induced by the Parkinson's disease (PD)-related neurotoxin, 6-hydroxydopamine (6-OHDA). Materials and methods: We determined the neuroprotective effects of EUE on 6-OHDA-induced neuronal cell death, cytotoxicity, reactive oxygen species (ROS) production, and mitochondrial membrane dysfunction. Moreover, we examined whether EUE suppressed phosphorylation of c-Jun N-terminal kinase (JNK), phosphatidylinositol 3-kinase (PI3K)/Akt, and glycogen synthase kinase-3 beta (GSK-3 beta). Furthermore, the neuroprotective effects of EUE on 6-OHDA-induced activation of nuclear factor-kappa B (NF-kappa B) was studied in SH-SY5Y cells. Results: Pretreatment of SH-SY5Y cells with EUE significantly reduced 6-OHDA-induced cell death and cytotoxicity. EUE inhibited 6-OHDA-induced generation of ROS, which conferred cytoprotection against 6-OHDA-induced oxidative injury. EUE treatment also strikingly inhibited 6-OHDA-induced mitochondrial dysfunction. In addition, EUE suppressed phosphorylation of JNK, PI3K/Akt, and GSK-3 beta. Furthermore, EUE blocked 6-OHDA-induced NF-kappa B nuclear translocation, an event downstream from JNK, PI3K/Akt, and GSK-3 beta phosphorylation. Moreover, chlorogenic acid (CGA), one of the active constituents of EUE, was also able to reduce 6-OHDA-induced toxicity in SH-SY5Y cells. Conclusion: Taken together, these results suggest that EUE attenuates oxidative stress through activation of JNK, PI3K/Akt, GSK-3 beta, and NF-kappa B pathways, thereby protecting cells from neuronal cell death. (C) 2014 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.relation.isPartOf | JOURNAL OF ETHNOPHARMACOLOGY | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | PARKINSONS-DISEASE MODEL | - |
dc.subject | DOPAMINERGIC-NEURONS | - |
dc.subject | CHLOROGENIC ACID | - |
dc.subject | PC12 CELLS | - |
dc.subject | ACTIVATION | - |
dc.subject | PROTECTS | - |
dc.subject | MPTP | - |
dc.subject | NEURODEGENERATION | - |
dc.subject | NEUROTOXICITY | - |
dc.title | Eucommia ulmoides Oliv. bark. attenuates 6-hydroxydopamine-induced neuronal cell death through inhibition of oxidative stress in SH-SY5Y cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000332356000016 | - |
dc.identifier.doi | 10.1016/j.jep.2013.12.048 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ETHNOPHARMACOLOGY, v.152, no.1, pp.173 - 182 | - |
dc.identifier.scopusid | 2-s2.0-84893875778 | - |
dc.citation.endPage | 182 | - |
dc.citation.startPage | 173 | - |
dc.citation.title | JOURNAL OF ETHNOPHARMACOLOGY | - |
dc.citation.volume | 152 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Kim, Sun Yeou | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Eucommia ulmoides Oliv. bark | - |
dc.subject.keywordAuthor | 6-Hydroxydopamine | - |
dc.subject.keywordAuthor | Oxidative stress | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Mitochondrial membrane dysfunction | - |
dc.subject.keywordAuthor | Parkinson&apos | - |
dc.subject.keywordAuthor | s disease | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | PARKINSONS-DISEASE MODEL | - |
dc.subject.keywordPlus | DOPAMINERGIC-NEURONS | - |
dc.subject.keywordPlus | CHLOROGENIC ACID | - |
dc.subject.keywordPlus | PC12 CELLS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PROTECTS | - |
dc.subject.keywordPlus | MPTP | - |
dc.subject.keywordPlus | NEURODEGENERATION | - |
dc.subject.keywordPlus | NEUROTOXICITY | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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