Role of Ca2(+)/Calmodulin-Dependent Kinase II-IRAK1 Interaction in LMP1-Induced NF-kappa B Activation
DC Field | Value | Language |
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dc.contributor.author | Kim, Jung-Eun | - |
dc.contributor.author | Kim, Sang Yong | - |
dc.contributor.author | Lim, Sue Yeon | - |
dc.contributor.author | Kieff, Elliott | - |
dc.contributor.author | Song, Yoon-Jae | - |
dc.date.available | 2020-02-28T18:42:15Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2014-02 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12867 | - |
dc.description.abstract | We have previously reported that interleukin-1 (IL-1) receptor-associated kinase (IRAK1) is essential for Epstein-Barr virus (EBV) latent infection membrane protein 1 (LMP1)-induced p65/RelA serine 536 phosphorylation and NF-kappa B activation but not for I kappa B kinase alpha (IKK alpha) or IKK beta activation (Y. J. Song, K. Y. Jen, V. Soni, E. Kieff, and E. Cahir-McFarland, Proc. Natl. Acad. Sci. U.S.A. 103: 2689-2694, 2006, doi: 10.1073/pnas.0511096103). Since the kinase activity of IRAK1 is not required for LMP1-induced NF-kappa B activation, IRAK1 is proposed to function as a scaffold protein to recruit a p65/RelA serine 536 kinase(s) to enhance NF-kappa B-dependent transcriptional activity. We now report that Ca2+/calmodulin-dependent protein kinase II (CaMKII) interacts with IRAK1 and is critical for LMP1-induced p65/RelA serine 536 phosphorylation and NF-kappa B activation. CaMKII bound the death domain of IRAK1 and directly phosphorylated p65/RelA at serine 536 in vitro. Downregulation of CaMKII activity or expression significantly reduced LMP1-induced p65/RelA serine 536 phosphorylation and NF-kappa B activation. Furthermore, LMP1-induced CaMKII activation and p65/RelA serine 536 phosphorylation were significantly reduced in IRAK1 knockout (KO) mouse embryonic fibroblasts (MEFs). Thus, IRAK1 may recruit and activate CaMKII, which phosphorylates p65/RelA serine 536 to enhance the transactivation potential of NF-kappa B in LMP1-induced NF-kappa B activation pathway. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.relation.isPartOf | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.title | Role of Ca2(+)/Calmodulin-Dependent Kinase II-IRAK1 Interaction in LMP1-Induced NF-kappa B Activation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000330582200004 | - |
dc.identifier.doi | 10.1128/MCB.00912-13 | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR BIOLOGY, v.34, no.3, pp.325 - 334 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84892453744 | - |
dc.citation.endPage | 334 | - |
dc.citation.startPage | 325 | - |
dc.citation.title | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.citation.volume | 34 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Kim, Jung-Eun | - |
dc.contributor.affiliatedAuthor | Kim, Sang Yong | - |
dc.contributor.affiliatedAuthor | Lim, Sue Yeon | - |
dc.contributor.affiliatedAuthor | Song, Yoon-Jae | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | EPSTEIN-BARR-VIRUS | - |
dc.subject.keywordPlus | LATENT MEMBRANE-PROTEIN | - |
dc.subject.keywordPlus | LYMPHOCYTE GROWTH TRANSFORMATION | - |
dc.subject.keywordPlus | RECEPTOR-ASSOCIATED KINASE-1 | - |
dc.subject.keywordPlus | EMBRYONAL CARCINOMA-CELLS | - |
dc.subject.keywordPlus | LYS63-LINKED POLYUBIQUITINATION | - |
dc.subject.keywordPlus | NEURITE OUTGROWTH | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | 1 ENGAGES | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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