Partial Purification and Properties of a Cysteine Protease from Citrus Red Mite Panonychus citri
- Authors
- Hong, Seong Chul; Her, Kyu-Hee; Kim, Heung-Up; Lee, Jaechun; Lee, Sang Pyo; Chung, Young-Bae
- Issue Date
- Feb-2014
- Publisher
- KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI
- Keywords
- Panonychus citri; mite; cysteine protease; allergen
- Citation
- KOREAN JOURNAL OF PARASITOLOGY, v.52, no.1, pp.117 - 120
- Journal Title
- KOREAN JOURNAL OF PARASITOLOGY
- Volume
- 52
- Number
- 1
- Start Page
- 117
- End Page
- 120
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12879
- DOI
- 10.3347/kjp.2014.52.1.117
- ISSN
- 0023-4001
- Abstract
- Several studies have reported that the citrus red mites Panonychus citri were an important allergen of citrus-cultivating farmers in Jeju Island. The aim of the present study was to purify and assess properties of a cysteine protease from the mites acting as a potentially pathogenic factor to citrus-cultivating farmers. A cysteine protease was purified using column chromatography of Mono Q anion exchanger and Superdex 200 HR gel filtration: It was estimated to be 46 kDa by gel filtration column chromatography and consisted of 2 polypeptides, at least. Cysteirie protease inhibitors, such as trans poxy-succinyl-L-Ieucyl-amido (4-guanidino) butane (E-64) and iodoacetic acid (IAA) totally inhibited the enzyme activities, whereas serine or metalloprotease inhibitors did not affect the activities. In addition, the purified enzyme degraded human IgG, collagen, and fibronectin, but not egg albumin. From these results, the cysteine protease of the mites might be involved in the pathogenesis such as tissue destruction and penetratibn instead of nutrient digestion.
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