Synthesis of 3 '-O-fluorescently mono-modified reversible terminators and their uses in sequencing-by-synthesis
DC Field | Value | Language |
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dc.contributor.author | Kim, Da-Rae | - |
dc.contributor.author | Kim, Taek-Soo | - |
dc.contributor.author | Kim, Eunsun | - |
dc.contributor.author | Min, Sun-Joon | - |
dc.contributor.author | Shin, Dongyun | - |
dc.contributor.author | Ahn, Dae-Ro | - |
dc.date.available | 2020-02-28T18:43:14Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2014-01-01 | - |
dc.identifier.issn | 0960-894X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12907 | - |
dc.description.abstract | Next-generation sequencing (NGS) technologies recently developed are now used for study of genomes from various organisms. Sequencing-by-synthesis (SBS) is a key strategy in the NGS. The SBS uses nucleotides so-called dual-modified reversible terminators (DRTs) in which bases are labeled with fluorophores and 3'-OH is protected with a reversibly cleavable chemical group, respectively. In this study, we examined the possibility of performing SBS with mono-modified reversible terminators (MRTs), in which the reversible blocking group on the 3'-OH plays a dual role as a fluorescent signal report as well as a chemical protection. We studied cyclic reversible termination by using two MRTs (dA and dT), wherein the modifications were two different fluorophores and cleavable to regenerate a free 3'-OH. We here demonstrated that SBS could be achieved with incorporation of MRTs by a DNA polymerase and correct base-calls based on the two different colors from the fluorophores. (C) 2013 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.relation.isPartOf | BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | - |
dc.subject | DNA-POLYMERASE | - |
dc.subject | TRIPHOSPHATES | - |
dc.subject | NUCLEOTIDES | - |
dc.title | Synthesis of 3 '-O-fluorescently mono-modified reversible terminators and their uses in sequencing-by-synthesis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000329114200039 | - |
dc.identifier.doi | 10.1016/j.bmcl.2013.11.040 | - |
dc.identifier.bibliographicCitation | BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, v.24, no.1, pp.209 - 213 | - |
dc.identifier.scopusid | 2-s2.0-84891491944 | - |
dc.citation.endPage | 213 | - |
dc.citation.startPage | 209 | - |
dc.citation.title | BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | - |
dc.citation.volume | 24 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Kim, Taek-Soo | - |
dc.contributor.affiliatedAuthor | Shin, Dongyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Sequencing-by-synthesis | - |
dc.subject.keywordAuthor | Reversible terminator | - |
dc.subject.keywordAuthor | Modified nucleotide | - |
dc.subject.keywordAuthor | DNA polymerase | - |
dc.subject.keywordAuthor | Triphosphate | - |
dc.subject.keywordPlus | DNA-POLYMERASE | - |
dc.subject.keywordPlus | TRIPHOSPHATES | - |
dc.subject.keywordPlus | NUCLEOTIDES | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Organic | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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