Asian Sand Dust Regulates IL-32 Production in Airway Epithelial Cells: Inhibitory Effect of Glucocorticoids
DC Field | Value | Language |
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dc.contributor.author | Shin, Jae-Min | - |
dc.contributor.author | Kim, Hwee-in | - |
dc.contributor.author | Park, Joo-Hoo | - |
dc.contributor.author | Hwang, You Jin | - |
dc.contributor.author | Lee, Heung-Man | - |
dc.date.available | 2020-02-27T02:41:52Z | - |
dc.date.created | 2020-02-04 | - |
dc.date.issued | 2019-07 | - |
dc.identifier.issn | 1945-8924 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1292 | - |
dc.description.abstract | Purpose Epidemiologic studies have reported that Asian sand dust (ASD) is associated with chronic inflammatory diseases of the respiratory system. Glucocorticoids (GCs) have potent anti-inflammatory properties. The aims of this study were to evaluate the effects of GCs on ASD-induced interleukin-32 (IL-32) expression and to identify the underlying signaling pathways in airway epithelial cells. Methods A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to evaluate cytotoxicity in A549 and human primary nasal epithelial cells. Expression levels of IL-32 messenger RNA and protein were measured by Western blot, real-time polymerase chain reaction, ELISA, and immunofluorescence staining. Signaling pathways were analyzed using specific inhibitors of Akt, MAPK, or NF-kappa B. The effects of GCs on the expression of ASD-induced IL-32 were confirmed with ex vivo organ cultures of the nasal interior turbinate. Results ASD (0-400 ng/mL) had no significant cytotoxic effects in A549 cells and human primary nasal epithelial cells. Expression levels of IL-32 were dose-dependently upregulated by ASD treatment in A549 cells. ASD induced phosphorylation of Akt, MAPK, and NF-kappa B, whereas GCs and specific inhibitors of Akt, MAPK, and NF-kappa B downregulated these activations and the expression of IL-32. These findings were further confirmed in human primary nasal epithelial cells and ex vivo organ cultures of the nasal interior turbinate. Conclusions GCs have an inhibitory effect on ASD-induced IL-32 expression via the Akt, MAPK, and NF-kappa B signaling pathways in airway epithelial cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SAGE PUBLICATIONS INC | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF RHINOLOGY & ALLERGY | - |
dc.subject | AMORPHOUS SILICA | - |
dc.subject | INTERLEUKIN-32 | - |
dc.subject | CYTOKINE | - |
dc.subject | EXPRESSION | - |
dc.subject | RHINITIS | - |
dc.title | Asian Sand Dust Regulates IL-32 Production in Airway Epithelial Cells: Inhibitory Effect of Glucocorticoids | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000475781800009 | - |
dc.identifier.doi | 10.1177/1945892419839538 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF RHINOLOGY & ALLERGY, v.33, no.4, pp.403 - 412 | - |
dc.identifier.scopusid | 2-s2.0-85063566161 | - |
dc.citation.endPage | 412 | - |
dc.citation.startPage | 403 | - |
dc.citation.title | AMERICAN JOURNAL OF RHINOLOGY & ALLERGY | - |
dc.citation.volume | 33 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Hwang, You Jin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Asian sand dust | - |
dc.subject.keywordAuthor | epithelial cell | - |
dc.subject.keywordAuthor | respiratory | - |
dc.subject.keywordAuthor | signaling pathway | - |
dc.subject.keywordAuthor | glucocorticoid | - |
dc.subject.keywordPlus | AMORPHOUS SILICA | - |
dc.subject.keywordPlus | INTERLEUKIN-32 | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RHINITIS | - |
dc.relation.journalResearchArea | Otorhinolaryngology | - |
dc.relation.journalWebOfScienceCategory | Otorhinolaryngology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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