Two dimensional array of ZnSe-ferritin for dopamine detection

Abstract

Less toxic zinc selenide (ZnSe) quantum dots were synthesized in the cavity of the apoferritin from horse spleen (HsAFr), and the two-dimensional ZnSe-ferritin nanodots were prepared on modified silicon surface. For utilizing the array as a biosensor, the photoluminescence (PL) spectrum change was investigated by accompanying its conjugation reaction with a neurotransmitter dopamine. When the array was conjugated with dopamine, a significant quenching of the fluorescence occurred and the PL intensity was a linear function of the dopamine concentration in the range of 0-0.6 mu M. Accordingly, the ZnSe-ferritin nanodot arrays can be employed as a useful sensing media for tiny concentration of dopamine. (C) 2014 The Japan Society of Applied Physics