2D planar PDMS microdevice attached onto a curved polycarbonate support for on-chip continuous-flow PCR employing a single heater

Abstract

With the development of microfluidic technology, many researchers have endeavored to miniaturize the polymerase chain reaction (PCR) process, which is considered one of the most time-consuming and labor-intensive but indispensible steps for enhancing the sensitivity of target detection. Here, we fabricate a 2D poly(dimethylsiloxane) (PDMS) microdevice attached onto a curved polycarbonate (PC). The curved PC shape is well sustained, and this allows an on-chip continuous-flow PCR to be performed employing a single heater. Two targets with amplicon sizes of 230 and 282 bp were successfully amplified within 30 min utilizing microdevices with 4° and 17° inclination angles, respectively. © 14CBMS.