黃連 추출물의 항염효능에 관한 연구

Abstract

Objectives : This research has been done to investigate the anti-inflammatory effect of Coptidis Rhizoma extracts. Method : Coptidis Rhizoma was extracted by 100℃ water. The extract (CC : Extract of Coptis chinensis rhizome) was used to examine its effects on the cell viability of mouse macrophage Raw 264.7 cell line. Also the production of nitric oxide (NO), the c-jun N-terminalkinase (JNK) activation and the production of cytokines such as (IL)-5 were evaluated in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. After the CC and LPS were applied to Raw 264.7 cells which were cultured for 24 hours, the MTT assay was performed. Result : The CC extracts didn't affect the viability of macrophage cells. However, the extracts inhibited the NO production and the JNK activation significantly in LPS-stimulated macrophage cells treated with 100 and 200 ㎍/mL concentrations. The CC extract, also, impeded the production of inflammation-related factors and cytokines such as KC, VEGF, MCP-1, GM-CSF, IL-1α, IL-5, IL-6, and IL-12p40 in LPS-stimulated macrophage cells at the concentration higher than 25 ㎍/mL. The production of basic-FGF concentration of 50 and 100 ㎍/mL, the production of IP-10 at 100 ㎍/mL, and the production of IFN-γ at 25 ㎍/mL, respectively. Conclusion : The CC prepared using 100℃ water showed the significant anti-inflammatory effect such as the inhibition not only on the production of NO, KC, VEGF, MCP-1, GM-CSF, IL-1α, IL-5, IL-6, and IL-12p40 in LPS-stimulated macrophage cells at or higher than the concentration of 25 ㎍/mL, but also on the JNK activation at 100 and 200 ㎍/mL.