Fully integrated and slidable paper-embedded plastic microdevice for point-of-care testing of multiple foodborne pathogens
DC Field | Value | Language |
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dc.contributor.author | Kieu The Loan Trinh | - |
dc.contributor.author | Thi Ngoc Diep Trinh | - |
dc.contributor.author | Lee, Nae Yoon | - |
dc.date.available | 2020-02-27T02:42:24Z | - |
dc.date.created | 2020-02-04 | - |
dc.date.issued | 2019-06 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1357 | - |
dc.description.abstract | This study presents a slidable paper-embedded plastic microdevice fully integrated with DNA extraction, loop-mediated isothermal amplification (LAMP), and colorimetric detection functionalities. The developed microdevice consists of three layers that allow a sliding movement to mix the sample and reagents for DNA purification, amplification, and detection in a sequential manner. An FTA card was employed in the main chamber for DNA extraction and purification from intact bacterial cells. Subsequently, LAMP reagents and fuchsin-stored chambers were pulled toward the main chambers for DNA amplifications at 65 degrees C. After 30 min, the detection reagents-stored chambers were then moved to main chambers for result analysis. For the detection of LAMP amplicons, a novel colorimetric fuchsin-based method was employed. The wide applicability of the integrated microdevice was demonstrated by successfully screening three major foodborne pathogens, namely Salmonella spp., Staphylococcus aureus, and Escherichia coli O157:H7 in food, enabling highly sensitive detection of 3.0 x 10(1) CFU/sample of Gram-negative bacteria (Salmonella spp. and Escherichia coli O157:H7) and 3.0 x 10(2) CFU/sample of Gram-positive bacteria (Staphylococcus aureus) within 75 min. The portable and integrated microdevice presented in this study holds significant promise for point-of-care applications to accurately and rapidly diagnose and control diseases. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.title | Fully integrated and slidable paper-embedded plastic microdevice for point-of-care testing of multiple foodborne pathogens | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000469157800016 | - |
dc.identifier.doi | 10.1016/j.bios.2019.04.011 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.135, pp.120 - 128 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85064252073 | - |
dc.citation.endPage | 128 | - |
dc.citation.startPage | 120 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 135 | - |
dc.contributor.affiliatedAuthor | Kieu The Loan Trinh | - |
dc.contributor.affiliatedAuthor | Thi Ngoc Diep Trinh | - |
dc.contributor.affiliatedAuthor | Lee, Nae Yoon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Integrated microdevice | - |
dc.subject.keywordAuthor | Foodborne pathogens | - |
dc.subject.keywordAuthor | DNA extraction | - |
dc.subject.keywordAuthor | Loop-mediated isothermal amplification (LAMP) | - |
dc.subject.keywordAuthor | Colorimetric detection | - |
dc.subject.keywordPlus | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | DNA PURIFICATION | - |
dc.subject.keywordPlus | DEVICE | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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