龍眼肉 물추출물이 대식세포의 염증반응과 Cytokine에 미치는 영향

Abstract

Objecives: The purpose of this study was to investigate the effects of LonganaeArillus water extract (LA) on the production of inflammatory mediators in RAW264.7 cell. LA is used for forgetfulness, insomnia, palpitation symptoms in koreanmedicine. Methods: In order to evaluate cytotoxicity of LA, cell viability was measured. To investigate anti-inflammatory effects of LA in the lipopolysacharide (LPS)-inducedRAW 264.7 cell, the concentration of nitric oxide (NO) and cytokines were measured. And when p-value is below 0.05, it is judged to have the significant difference statistically. Results:1. LA showed no cytotoxicity. 2. LA inhibited significantly the production on NO at the concentration of 50and 200 μg/ml. 3. LA inhibited significantly the production on interleukin (IL)-1β at the concentrationof 25, 50, 100 and 200 μg/ml. 4. LA inhibited significantly the production on tumor necrosis factor (TNF)-αat the concentration of 50, 100 and 200 μg/ml. 5. LA inhibited significantly the production on lipopolysaccharide-induced chemokine(LIX) at the concentration of 25, 100 and 200 μg/ml. 6. LA inhibited significantly the production on regulated on activation normalT cell expressed and secreted (RANTES) at the concentration of 200 μg/ml. Conclusions: These results suggest that LA has anti-inflammatory effect.