위령선 추출물이 Human Neuroblastoma 세포주에서 CoCl2에 의해 유도된 세포사멸에 미치는 보호효과
DC Field | Value | Language |
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dc.contributor.author | 박정우 | - |
dc.contributor.author | 임형호 | - |
dc.date.available | 2020-02-28T21:41:26Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1229-1854 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13742 | - |
dc.description.abstract | Objectives The purpose of this study was to evaluate the effects of Clematidis radix extract on CoCl2-induced apoptosis in SH-SY5Y human neuroblastoma cells. Methods In order to investigate the protective effect of Clematidis radix on CoCl2-induced cytotoxicity in neuronal cells, MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI(4,6-diamidino-2-phenylindoleI) staining, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) assay, DNA fragmentation assay and western blotting were performed on SH-SY5Y human neuroblastoma cells. Results Cells treated with CoCl2 exhibited several apoptotic features, while cells pre-treated with Clematidis radix prior to CoCl2 exposure showed a decrease in the occurrence of apoptotic features. CoCl2 increased HIF-1α expression, in contrast, Clematidis radix treatment decreased CoCl2-induced HIF-1α expression. Pre-treatment with the extract of Clematidis radix suppressed Bax, cytochrome c, and caspase-3 expressions, and also increased Bcl-2 expression in SH-SY5Y human neuroblastoma cells. Conclusions These results suggest that Clematidis radix may exert a protective effect on CoCl2-induced apoptosis in SH-SY5Y human neuroblastoma cells. | - |
dc.language | 한국어 | - |
dc.language.iso | ko | - |
dc.publisher | 한방재활의학과학회 | - |
dc.relation.isPartOf | 한방재활의학과학회지 | - |
dc.title | 위령선 추출물이 Human Neuroblastoma 세포주에서 CoCl2에 의해 유도된 세포사멸에 미치는 보호효과 | - |
dc.title.alternative | Protective Effect of Clematidis Radix Extract on CoCl2-induced Apoptosis in Human Neuroblastoma Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | 한방재활의학과학회지, v.24, no.2, pp.41 - 50 | - |
dc.identifier.kciid | ART001869160 | - |
dc.citation.endPage | 50 | - |
dc.citation.startPage | 41 | - |
dc.citation.title | 한방재활의학과학회지 | - |
dc.citation.volume | 24 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | 박정우 | - |
dc.contributor.affiliatedAuthor | 임형호 | - |
dc.subject.keywordAuthor | Clematidis radix | - |
dc.subject.keywordAuthor | CoCl2 | - |
dc.subject.keywordAuthor | Hypoxia | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | SH-SY5Y human neuro-blastoma cells | - |
dc.description.journalRegisteredClass | kci | - |
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