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위령선 추출물이 Human Neuroblastoma 세포주에서 CoCl2에 의해 유도된 세포사멸에 미치는 보호효과

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dc.contributor.author박정우-
dc.contributor.author임형호-
dc.date.available2020-02-28T21:41:26Z-
dc.date.created2020-02-12-
dc.date.issued2014-
dc.identifier.issn1229-1854-
dc.identifier.urihttps://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13742-
dc.description.abstractObjectives The purpose of this study was to evaluate the effects of Clematidis radix extract on CoCl2-induced apoptosis in SH-SY5Y human neuroblastoma cells. Methods In order to investigate the protective effect of Clematidis radix on CoCl2-induced cytotoxicity in neuronal cells, MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI(4,6-diamidino-2-phenylindoleI) staining, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) assay, DNA fragmentation assay and western blotting were performed on SH-SY5Y human neuroblastoma cells. Results Cells treated with CoCl2 exhibited several apoptotic features, while cells pre-treated with Clematidis radix prior to CoCl2 exposure showed a decrease in the occurrence of apoptotic features. CoCl2 increased HIF-1α expression, in contrast, Clematidis radix treatment decreased CoCl2-induced HIF-1α expression. Pre-treatment with the extract of Clematidis radix suppressed Bax, cytochrome c, and caspase-3 expressions, and also increased Bcl-2 expression in SH-SY5Y human neuroblastoma cells. Conclusions These results suggest that Clematidis radix may exert a protective effect on CoCl2-induced apoptosis in SH-SY5Y human neuroblastoma cells.-
dc.language한국어-
dc.language.isoko-
dc.publisher한방재활의학과학회-
dc.relation.isPartOf한방재활의학과학회지-
dc.title위령선 추출물이 Human Neuroblastoma 세포주에서 CoCl2에 의해 유도된 세포사멸에 미치는 보호효과-
dc.title.alternativeProtective Effect of Clematidis Radix Extract on CoCl2-induced Apoptosis in Human Neuroblastoma Cells-
dc.typeArticle-
dc.type.rimsART-
dc.description.journalClass2-
dc.identifier.bibliographicCitation한방재활의학과학회지, v.24, no.2, pp.41 - 50-
dc.identifier.kciidART001869160-
dc.citation.endPage50-
dc.citation.startPage41-
dc.citation.title한방재활의학과학회지-
dc.citation.volume24-
dc.citation.number2-
dc.contributor.affiliatedAuthor박정우-
dc.contributor.affiliatedAuthor임형호-
dc.subject.keywordAuthorClematidis radix-
dc.subject.keywordAuthorCoCl2-
dc.subject.keywordAuthorHypoxia-
dc.subject.keywordAuthorApoptosis-
dc.subject.keywordAuthorSH-SY5Y human neuro-blastoma cells-
dc.description.journalRegisteredClasskci-
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