Bee Venom Enhanced Cytotoxic Effect of Natural Killer Cells on Human Lung Cancer Through Inducing Extrinsic Apoptosis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung Hyun Kim | - |
dc.contributor.author | 송호섭 | - |
dc.date.available | 2020-02-28T21:44:53Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 2586-288X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13967 | - |
dc.description.abstract | Objectives : I investigated whether Bee Venom can synergistically strengthen the cytotoxic effects of NK-92 cells, enhancing the inhibition of the growth of Lung Cancer Cells including A549 and NCI-H460 through induction of death receptor dependent extrinsic apoptosis and NO generation in the Nitro-oxide pathway. Methods : Bee Venom inhibited cell proliferation of A549 or NCI-H460 Human Lung Cancer Cells as well as NK-92 Cells. Moreover, when they were co-punctured with NK cells and concomitantly treated by 3 ㎍/㎖ of Bee Venom, more influence was exerted on inhibition of proliferation of A549 or NCI-H460 Human Lung Cancer Cells than BV or NK cell co-culture alone. Results : The expression of Fas, TNFR2, DR3, DR6 in A549 Lung Cancer Cells was significantly increased by co-culture of NK-92 cells and treatment of 3 ㎍/㎖ of Bee Venom, compared to co-culture of NK-92 cells alone, whereas the expression of Fas, TNFR2, DR6 in NCI-H460 Lung Cancer Cells was significantly increased by co-culture of NK-92 cells, representing no synergistic effects in the co-culture of NK-92 cell and concomitant treatment of 3 ㎍/㎖ of Bee Venom. Coincidently, caspase-8, a expression of pro-apoptotic proteins in the extrinsic apoptosis pathway demonstrated same results as the above. Meanwhile, In NO generation, there is little change of NO generation in co-culture of NK-92 cells with A549 cells as well as the co-culture of NK-92 cell with them and concomitant treatment of 3 ㎍/㎖ of Bee Venom, whereas increase of NO generation was shown in co-culture of NK-92 cells with NCI-H460 cells as well as the co-culture of NK-92 cell with them and concomitant treatment of 3 ㎍/㎖ of Bee Venom, although synergistic effects by Bee Venom was not found. Conclusions : These present data provide that Bee Venom could be useful candidate compounds to enhance lung cancer growth inhibiting ability of NK-92 cells through DR expression and the related apoptosis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 대한침구의학회 | - |
dc.relation.isPartOf | Journal of Acupuncture Research | - |
dc.title | Bee Venom Enhanced Cytotoxic Effect of Natural Killer Cells on Human Lung Cancer Through Inducing Extrinsic Apoptosis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | Journal of Acupuncture Research, v.31, no.1, pp.111 - 119 | - |
dc.identifier.kciid | ART001858916 | - |
dc.citation.endPage | 119 | - |
dc.citation.startPage | 111 | - |
dc.citation.title | Journal of Acupuncture Research | - |
dc.citation.volume | 31 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | 송호섭 | - |
dc.subject.keywordAuthor | Bee venom | - |
dc.subject.keywordAuthor | Lung cancer | - |
dc.subject.keywordAuthor | A549 | - |
dc.subject.keywordAuthor | NCI-H460 | - |
dc.subject.keywordAuthor | NK-92 | - |
dc.subject.keywordAuthor | Death receptor | - |
dc.subject.keywordAuthor | Nitric oxide(NO) | - |
dc.description.journalRegisteredClass | kci | - |
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