Characteristics of coliphage ECP4 and potential use as a sanitizing agent for biocontrol of Escherichia coli O157:H7
- Authors
- Lee, Young-Duck; Kim, Jin-Young; Park, Jong-Hyun
- Issue Date
- Dec-2013
- Publisher
- ELSEVIER SCI LTD
- Keywords
- E. coli O157:H7; Coliphage; Biocontrol; Biofilm
- Citation
- FOOD CONTROL, v.34, no.2, pp.255 - 260
- Journal Title
- FOOD CONTROL
- Volume
- 34
- Number
- 2
- Start Page
- 255
- End Page
- 260
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14062
- DOI
- 10.1016/j.foodcont.2013.04.043
- ISSN
- 0956-7135
- Abstract
- Escherichia coli O157:H7 is an important pathogenic bacterium to humans because it produces various toxins, such as shiga-toxin. Coliphage ECP4, which belongs to the Siphoviridae family, was isolated from bovine feces to test its utility as a potential agent for the biocontrol of E. coli O157:H7. The burst size of coliphage ECP4 was about 80 PFU/cell, after a latent period of 30-35 min. Coliphage ECP4 was susceptible to temperatures above 70 degrees C; however, its stability was slightly reduced to 1-2 log PFU/ml after 30 min in 70% ethanol. In addition, the shiga toxin gene was not detected on coliphage ECP4. Coliphage ECP4 inhibited the growth of E. coli O157:H7 in vegetable juice, and was not detected in cabbage after 5 h. When coliphage ECP4 was applied to biofilm-formed E. coli O157:H7, E. coli O157:H7 was efficiently reduced. The newly identified coliphage ECP4 might effectively reduce E. coli O157:H7 or its biofilmed-form. Therefore, the coliphage ECP4 might be an efficient sanitizer for fresh produce contaminated with E. coli O157:H7 in the biofilm environment. (C) 2013 Elsevier Ltd. All rights reserved.
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