Influence of DNA Concentration on the Interfacial Electrode Impedance
DC Field | Value | Language |
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dc.contributor.author | Cho, Sungbo | - |
dc.contributor.author | Oh, Younshin | - |
dc.contributor.author | Ahn, Sung-Min | - |
dc.date.available | 2020-02-28T22:44:14Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-11 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14179 | - |
dc.description.abstract | Sensitive and accurate DNA quantification is an essential step in most DNA analyses, especially in analysis of a small amount of DNA. Instead of typical DNA quantification methods (i.e., UV spectrometry, fluorometry, and quantitative polymerase chain reaction), electrical impedance spectroscopy has recently been investigated for label-free and efficient quantification of DNA. In this study, quantification of DNA in solution was investigated using electrical impedance spectroscopy without pre-processing, including the modification of the electrode surface and immobilization of DNA onto the electrode. The results showed that the measured impedance spectra were in agreement with a designed equivalent circuit model, and that the extrapolated parameters of interfacial electrode impedance were dependent on DNA concentration. The impedance measured at 1 and 10 kHz showed good correlation with DNA concentration (R-2 > 0.95), with a dynamic range of detection from 0.01 pg/mu l to 100 ng/mu l. Based on the results, it is expected to utilize impedance spectroscopy for the real-time and label-free characterization of DNA concentration with high sensitivity and low operation cost. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.relation.isPartOf | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.subject | REAL-TIME | - |
dc.subject | TRANSPORT | - |
dc.subject | BIOSENSOR | - |
dc.subject | HYBRIDIZATION | - |
dc.subject | SPECTROSCOPY | - |
dc.subject | QUANTITATION | - |
dc.title | Influence of DNA Concentration on the Interfacial Electrode Impedance | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000328706800015 | - |
dc.identifier.doi | 10.1166/jnn.2013.8085 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.13, no.11, pp.7291 - 7294 | - |
dc.identifier.scopusid | 2-s2.0-84891498654 | - |
dc.citation.endPage | 7294 | - |
dc.citation.startPage | 7291 | - |
dc.citation.title | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.volume | 13 | - |
dc.citation.number | 11 | - |
dc.contributor.affiliatedAuthor | Cho, Sungbo | - |
dc.contributor.affiliatedAuthor | Oh, Younshin | - |
dc.contributor.affiliatedAuthor | Ahn, Sung-Min | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | DNA Quantification | - |
dc.subject.keywordAuthor | Equivalent Circuit | - |
dc.subject.keywordAuthor | Fitting Analysis | - |
dc.subject.keywordAuthor | Impedance Spectroscopy | - |
dc.subject.keywordPlus | REAL-TIME | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | HYBRIDIZATION | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | QUANTITATION | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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