Daidzein causes cytochrome c-mediated apoptosis via the Bcl-2 family in human hepatic cancer cells
- Authors
- Park, Hyun Jin; Jeon, Young Keul; You, Dong Hun; Nam, Myeong Jin
- Issue Date
- Oct-2013
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Liver cancer; Apoptosis; Daidzein; Proxiredoxin 3
- Citation
- FOOD AND CHEMICAL TOXICOLOGY, v.60, pp.542 - 549
- Journal Title
- FOOD AND CHEMICAL TOXICOLOGY
- Volume
- 60
- Start Page
- 542
- End Page
- 549
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14263
- DOI
- 10.1016/j.fct.2013.08.022
- ISSN
- 0278-6915
- Abstract
- Daidzein, which belongs to the group of isoflavones from soybeans, has been extensively researched prostate, cervix, brain, breast, and colon cancer cell lines. However, daidzein has not been thoroughly investigated in human hepatic cancer cells; therefore, we investigated whether it inhibits hepatic cancer cell growth. Decreased cell proliferation was measured in daidzein-treated hepatic cancer cells (SK-HEP-1) upon real-time cell electronic sensing analysis however, it was not affected on normal human hepatocytes (Chang). Daidzein-induced apoptosis was demonstrated by comet and TUNEL assay. Moreover, we conducted two-dimensional electrophoresis to study the mechanism of daidzein-induced apoptosis in daidzein-treated SK-HEP-1 cells. Expression of peroxiredoxin-3 (Prdx-3), which modulates redox homeostasis of cells, was increased in protein analysis. Additionally, we measured the levels of reactive oxygen species and it was decreased in daidzein-treated SKHEP-1 cells. Daidzein-induced apoptosis in SK-HEP-1 cells was also associated with the up-regulation of Bak and down-regulation of Bcl-2 and Bcl-xL proteins. Moreover, daidzein treatment increased in the release of mitochondrial cytochrome c and activation of APAF-1, caspase 9 and caspase 3. Overall, these result indicate that daidzein is a potent inducer of apoptosis in hepatic cancer cells via mitochondrial pathway. (C) 2013 Elsevier Ltd. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 생명과학과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.