Proteinase 3 induces oxidative stress-mediated neuronal death in rat primary cortical neuron
DC Field | Value | Language |
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dc.contributor.author | Kwon, Kyoung Ja | - |
dc.contributor.author | Cho, Kyu Suk | - |
dc.contributor.author | Kim, Jung Nam | - |
dc.contributor.author | Kim, Min Kyeong | - |
dc.contributor.author | Lee, Eun Joo | - |
dc.contributor.author | Kim, Soo Young | - |
dc.contributor.author | Jeon, Se Jin | - |
dc.contributor.author | Kim, Ki Chan | - |
dc.contributor.author | Han, Jeong Eun | - |
dc.contributor.author | Kang, Young Sun | - |
dc.contributor.author | Kim, Soohyun | - |
dc.contributor.author | Kim, Hahn Young | - |
dc.contributor.author | Han, Seol-Heui | - |
dc.contributor.author | Bahn, Geonho | - |
dc.contributor.author | Choi, Ji Woong | - |
dc.contributor.author | Shin, Chan Young | - |
dc.date.available | 2020-02-28T23:42:47Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-08-26 | - |
dc.identifier.issn | 0304-3940 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14370 | - |
dc.description.abstract | The recruitment of neutrophils into the cerebral microcirculation occurs, especially, in acute brain diseases like a focal cerebral ischemia and plays important role in pathological processes. Proteinase 3 is one of the three major proteinases expressed in neutrophils but no reports are available whether proteinase 3 can modulate neuronal survival. In this study, treatment of cultured rat primary cortical neuron with proteinase 3 induced overt reactive oxygen species production and decreased total glutathione contents as well as disruption of mitochondrial transmembrane potential. Proteinase 3 induced neuronal cell death as evidenced by MTT analysis as well as propidium iodide staining, which was prevented by pretreatment with an antioxidant, N-acetyl cysteine. Proteinase 3 increased activation of procaspase-3 and altered expression level of apoptotic regulator proteins, such as Bcl-2, Bax, and Bcl-xL. Similar to in vitro data, a direct microinjection of proteinase 3 into striatum of rat brain induced neuronal death, which was mediated by reactive oxygen species. These results suggest that proteinase 3 is new essential regulator of neuronal cell death pathway in a condition of excess neutrophil encounter in neuroinflammatory conditions. (C) 2013 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.relation.isPartOf | NEUROSCIENCE LETTERS | - |
dc.subject | NEUTROPHIL-DERIVED PROTEINASE-3 | - |
dc.subject | ELASTASE | - |
dc.subject | CELLS | - |
dc.subject | ISCHEMIA | - |
dc.subject | CLEAVAGE | - |
dc.subject | ADHESION | - |
dc.subject | RELEASE | - |
dc.subject | DAMAGE | - |
dc.title | Proteinase 3 induces oxidative stress-mediated neuronal death in rat primary cortical neuron | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000322092000013 | - |
dc.identifier.doi | 10.1016/j.neulet.2013.05.060 | - |
dc.identifier.bibliographicCitation | NEUROSCIENCE LETTERS, v.548, pp.67 - 72 | - |
dc.identifier.scopusid | 2-s2.0-84880132505 | - |
dc.citation.endPage | 72 | - |
dc.citation.startPage | 67 | - |
dc.citation.title | NEUROSCIENCE LETTERS | - |
dc.citation.volume | 548 | - |
dc.contributor.affiliatedAuthor | Han, Jeong Eun | - |
dc.contributor.affiliatedAuthor | Choi, Ji Woong | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Neutrophil | - |
dc.subject.keywordAuthor | Proteinase 3 | - |
dc.subject.keywordAuthor | Neuron | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordPlus | NEUTROPHIL-DERIVED PROTEINASE-3 | - |
dc.subject.keywordPlus | ELASTASE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ISCHEMIA | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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