Determination of proline-hydroxyproline dipeptide in rat urine by high-performance anion-exchange chromatography coupled with pulsed amperometric detection
DC Field | Value | Language |
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dc.contributor.author | Sim, Hee-Jung | - |
dc.contributor.author | Moon, Eunjung | - |
dc.contributor.author | Kim, Sun Yeou | - |
dc.contributor.author | Hong, Seon-Pyo | - |
dc.date.available | 2020-02-28T23:44:15Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-07-01 | - |
dc.identifier.issn | 1570-0232 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14431 | - |
dc.description.abstract | A method for determination of proline-hydroxyproline dipeptide (PHP) was developed using high-performance anion-exchange chromatography-pulsed amperometric detection (HPAEC-PAD). This method resulted in good separation of proline (Pro), hydroxyproline (Hyp), and PHP within 20 min using a mobile phase of 1.2 mM Ba(OH)(2)+ 1.5 mM Ba(OAc)(2). The linear dynamic ranges and their detection limits (S/N = 3) were 1-100 (r(2) = 0.9990-0.9999) and 0.05-0.3 mu M, respectively. Mean recoveries were 91.6-121.3% and 92.2-110.3% for intra- and inter-day assays, respectively. Our HPAEC-PAD method showed clear differences in the corrected PHP levels measured in urine samples from two groups of rats, sham-operated and ovariectomized, without the need for prior acid hydrolysis or sample derivatization. (C) 2013 Elsevier B.V. All rights reserved, | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | - |
dc.subject | BONE-RESORPTION | - |
dc.subject | HPAEC-PAD | - |
dc.subject | HYDROLYSIS | - |
dc.subject | TURNOVER | - |
dc.subject | MARKERS | - |
dc.title | Determination of proline-hydroxyproline dipeptide in rat urine by high-performance anion-exchange chromatography coupled with pulsed amperometric detection | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000320744800011 | - |
dc.identifier.doi | 10.1016/j.jchromb.2013.04.033 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.930, pp.70 - 74 | - |
dc.identifier.scopusid | 2-s2.0-84878878709 | - |
dc.citation.endPage | 74 | - |
dc.citation.startPage | 70 | - |
dc.citation.title | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | - |
dc.citation.volume | 930 | - |
dc.contributor.affiliatedAuthor | Moon, Eunjung | - |
dc.contributor.affiliatedAuthor | Kim, Sun Yeou | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Anion-exchange chromatography | - |
dc.subject.keywordAuthor | Pulsed amperometric detection | - |
dc.subject.keywordAuthor | Barium hydroxide | - |
dc.subject.keywordAuthor | Proline-hydroxyproline | - |
dc.subject.keywordAuthor | Hydroxyproline | - |
dc.subject.keywordPlus | BONE-RESORPTION | - |
dc.subject.keywordPlus | HPAEC-PAD | - |
dc.subject.keywordPlus | HYDROLYSIS | - |
dc.subject.keywordPlus | TURNOVER | - |
dc.subject.keywordPlus | MARKERS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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